Development of an RNA interference (RNAi) gene knockdown protocol in the anaerobic gut fungus Pecoramyces ruminantium strain C1A

Development of an RNA interference (RNAi) gene knockdown protocol in the anaerobic gut fungus Pecoramyces ruminantium strain C1A

Members of the anaerobic gut fungi (AGF) reside in rumen, hindgut, and feces of ruminant and non-ruminant herbivorous mammals and reptilian herbivores. No protocols for gene insertion, deletion, silencing, or mutation are currently available for the AGF, rendering gene-targeted molecular biological...

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Bibliographic Details
Main Authors: Shelby S. Calkins, Nicole C. Elledge, Katherine E. Mueller, Stephen M. Marek, MB Couger, Mostafa S. Elshahed, Noha H. Youssef
Format: Article
Language:English
Published: PeerJ Inc. 2018-01-01
Series:PeerJ
Subjects:
RNA interference
Pecoramyces ruminantium
D-lactate dehydrogenase
Anaerobic gut fungi
Online Access:https://peerj.com/articles/4276.pdf

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https://peerj.com/articles/4276.pdf

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