Drosophila Hrp48 Is Required for Mushroom Body Axon Growth, Branching and Guidance.

RNA binding proteins assemble on mRNAs to control every single step of their life cycle, from nuclear splicing to cytoplasmic localization, stabilization or translation. Consistent with an essential role of RNA binding proteins in neuronal maturation and function, mutations in this class of proteins...

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Main Authors: Hélène Bruckert, Giovanni Marchetti, Mirana Ramialison, Florence Besse
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4551846?pdf=render
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spelling doaj-7978f831dae84c69a84bb06a7118e55b2020-11-24T21:08:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01108e013661010.1371/journal.pone.0136610Drosophila Hrp48 Is Required for Mushroom Body Axon Growth, Branching and Guidance.Hélène BruckertGiovanni MarchettiMirana RamialisonFlorence BesseRNA binding proteins assemble on mRNAs to control every single step of their life cycle, from nuclear splicing to cytoplasmic localization, stabilization or translation. Consistent with an essential role of RNA binding proteins in neuronal maturation and function, mutations in this class of proteins, in particular in members of the hnRNP family, have been associated with neurological diseases. To date, however, the physiological function of hnRNPs during in vivo neuronal development has remained poorly explored. Here, we have investigated the role of Drosophila Hrp48, a fly homologue of mammalian hnRNP A2/B1, during central nervous system development. Using a combination of mutant conditions, we showed that hrp48 is required for the formation, growth and guidance of axonal branches in Mushroom Body neurons. Furthermore, our results revealed that hrp48 inactivation induces an overextension of Mushroom Body dorsal axonal branches, with a significantly higher penetrance in females than in males. Finally, as demonstrated by immunolocalization studies, Hrp48 is confined to Mushroom Body neuron cell bodies, where it accumulates in the cytoplasm from larval stages to adulthood. Altogether, our data provide evidence for a crucial in vivo role of the hnRNP Hrp48 in multiple aspects of axon guidance and branching during nervous system development. They also indicate cryptic sex differences in the development of sexually non-dimorphic neuronal structures.http://europepmc.org/articles/PMC4551846?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Hélène Bruckert
Giovanni Marchetti
Mirana Ramialison
Florence Besse
spellingShingle Hélène Bruckert
Giovanni Marchetti
Mirana Ramialison
Florence Besse
Drosophila Hrp48 Is Required for Mushroom Body Axon Growth, Branching and Guidance.
PLoS ONE
author_facet Hélène Bruckert
Giovanni Marchetti
Mirana Ramialison
Florence Besse
author_sort Hélène Bruckert
title Drosophila Hrp48 Is Required for Mushroom Body Axon Growth, Branching and Guidance.
title_short Drosophila Hrp48 Is Required for Mushroom Body Axon Growth, Branching and Guidance.
title_full Drosophila Hrp48 Is Required for Mushroom Body Axon Growth, Branching and Guidance.
title_fullStr Drosophila Hrp48 Is Required for Mushroom Body Axon Growth, Branching and Guidance.
title_full_unstemmed Drosophila Hrp48 Is Required for Mushroom Body Axon Growth, Branching and Guidance.
title_sort drosophila hrp48 is required for mushroom body axon growth, branching and guidance.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2015-01-01
description RNA binding proteins assemble on mRNAs to control every single step of their life cycle, from nuclear splicing to cytoplasmic localization, stabilization or translation. Consistent with an essential role of RNA binding proteins in neuronal maturation and function, mutations in this class of proteins, in particular in members of the hnRNP family, have been associated with neurological diseases. To date, however, the physiological function of hnRNPs during in vivo neuronal development has remained poorly explored. Here, we have investigated the role of Drosophila Hrp48, a fly homologue of mammalian hnRNP A2/B1, during central nervous system development. Using a combination of mutant conditions, we showed that hrp48 is required for the formation, growth and guidance of axonal branches in Mushroom Body neurons. Furthermore, our results revealed that hrp48 inactivation induces an overextension of Mushroom Body dorsal axonal branches, with a significantly higher penetrance in females than in males. Finally, as demonstrated by immunolocalization studies, Hrp48 is confined to Mushroom Body neuron cell bodies, where it accumulates in the cytoplasm from larval stages to adulthood. Altogether, our data provide evidence for a crucial in vivo role of the hnRNP Hrp48 in multiple aspects of axon guidance and branching during nervous system development. They also indicate cryptic sex differences in the development of sexually non-dimorphic neuronal structures.
url http://europepmc.org/articles/PMC4551846?pdf=render
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