Optical Bioimaging: From Living Tissue to a Single Molecule: Optical Detection of Synaptically Induced Glutamate Transporter Activity in Hippocampal Slices

Optical Bioimaging: From Living Tissue to a Single Molecule: Optical Detection of Synaptically Induced Glutamate Transporter Activity in Hippocampal Slices

ABSTRACT: Since voltage-sensitive dyes (VSDs) change their optical properties in response to membrane potential changes, non-invasive optical measurements of membrane excitation using VSDs have been applied to various types of excitable tissue. This article deals with the authors' recent work u...

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Main Authors: Takeshi Nakamura, Yoshinobu Kawamura, Hiroyoshi Miyakawa
Format: Article
Language:English
Published: Elsevier 2003-01-01
Series:Journal of Pharmacological Sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S1347861319325708
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spelling doaj-796dfba58f6a4dbc9af9078ab003fce02020-11-25T01:48:50ZengElsevierJournal of Pharmacological Sciences1347-86132003-01-01933234241Optical Bioimaging: From Living Tissue to a Single Molecule: Optical Detection of Synaptically Induced Glutamate Transporter Activity in Hippocampal SlicesTakeshi Nakamura0Yoshinobu Kawamura1Hiroyoshi Miyakawa2Calcium Oscillation Project, ICORP, Japan Science and Technology Corporation, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, JapanLaboratory of Cellular Neurobiology, School of Life Science, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji-shi, Tokyo 192-0392, JapanLaboratory of Cellular Neurobiology, School of Life Science, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji-shi, Tokyo 192-0392, JapanABSTRACT: Since voltage-sensitive dyes (VSDs) change their optical properties in response to membrane potential changes, non-invasive optical measurements of membrane excitation using VSDs have been applied to various types of excitable tissue. This article deals with the authors' recent work using this technique in addition to basic and technical information about VSD imaging. By applying this technology to hippocampal brain slices, we have found that astrocytes play indispensable roles in sequestering glutamate, maintenance of synaptic transmission, and neuronal survival. Finally, the potential of VSD imaging for drug development was discussed.http://www.sciencedirect.com/science/article/pii/S1347861319325708
collection DOAJ
language English
format Article
sources DOAJ
author Takeshi Nakamura
Yoshinobu Kawamura
Hiroyoshi Miyakawa
spellingShingle Takeshi Nakamura
Yoshinobu Kawamura
Hiroyoshi Miyakawa
Optical Bioimaging: From Living Tissue to a Single Molecule: Optical Detection of Synaptically Induced Glutamate Transporter Activity in Hippocampal Slices
Journal of Pharmacological Sciences
author_facet Takeshi Nakamura
Yoshinobu Kawamura
Hiroyoshi Miyakawa
author_sort Takeshi Nakamura
title Optical Bioimaging: From Living Tissue to a Single Molecule: Optical Detection of Synaptically Induced Glutamate Transporter Activity in Hippocampal Slices
title_short Optical Bioimaging: From Living Tissue to a Single Molecule: Optical Detection of Synaptically Induced Glutamate Transporter Activity in Hippocampal Slices
title_full Optical Bioimaging: From Living Tissue to a Single Molecule: Optical Detection of Synaptically Induced Glutamate Transporter Activity in Hippocampal Slices
title_fullStr Optical Bioimaging: From Living Tissue to a Single Molecule: Optical Detection of Synaptically Induced Glutamate Transporter Activity in Hippocampal Slices
title_full_unstemmed Optical Bioimaging: From Living Tissue to a Single Molecule: Optical Detection of Synaptically Induced Glutamate Transporter Activity in Hippocampal Slices
title_sort optical bioimaging: from living tissue to a single molecule: optical detection of synaptically induced glutamate transporter activity in hippocampal slices
publisher Elsevier
series Journal of Pharmacological Sciences
issn 1347-8613
publishDate 2003-01-01
description ABSTRACT: Since voltage-sensitive dyes (VSDs) change their optical properties in response to membrane potential changes, non-invasive optical measurements of membrane excitation using VSDs have been applied to various types of excitable tissue. This article deals with the authors' recent work using this technique in addition to basic and technical information about VSD imaging. By applying this technology to hippocampal brain slices, we have found that astrocytes play indispensable roles in sequestering glutamate, maintenance of synaptic transmission, and neuronal survival. Finally, the potential of VSD imaging for drug development was discussed.
url http://www.sciencedirect.com/science/article/pii/S1347861319325708
work_keys_str_mv AT takeshinakamura opticalbioimagingfromlivingtissuetoasinglemoleculeopticaldetectionofsynapticallyinducedglutamatetransporteractivityinhippocampalslices
AT yoshinobukawamura opticalbioimagingfromlivingtissuetoasinglemoleculeopticaldetectionofsynapticallyinducedglutamatetransporteractivityinhippocampalslices
AT hiroyoshimiyakawa opticalbioimagingfromlivingtissuetoasinglemoleculeopticaldetectionofsynapticallyinducedglutamatetransporteractivityinhippocampalslices
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