Detection of Abnormal Extracellular Matrix in the Interstitium of Regenerating Renal Tubules

• Main Authors: Will W. Minuth, Lucia Denk
• Format: Article
• Language: English
• Published: MDPI AG 2014-12-01
• Series: International Journal of Molecular Sciences
• Subjects: kidney; stem/progenitor cells; abnormal development; extracellular matrix; glutaraldehyde; cupromeronic blue; ruthenium red; tannic acid; transmission electron microscopy
• Online Access: http://www.mdpi.com/1422-0067/15/12/23240

Stem/progenitor cells are promising candidates for the regeneration of parenchyma in acute and chronic renal failure. However, recent data exhibit that survival of stem/progenitor cells after implantation in diseased renal parenchyma is restricted. To elaborate basic parameters improving survival, cell seeding was simulated under advanced in vitro conditions. After isolation, renal stem/progenitor cells were mounted in a polyester interstitium for perfusion culture. During generation of tubules, chemically defined CO2 Independent Medium or Leibovitz’s L-15 Medium was applied. Specimens were then fixed for transmission electron microscopy to analyze morphological features in generated tubules. Fixation in conventional glutaraldehyde (GA) solution shows development of tubules each exhibiting a polarized epithelium, an intact basal lamina and an inconspicuous interstitium. In contrast, special fixation of specimens in GA solution containing cupromeronic blue, ruthenium red or tannic acid unveils previously not visible extracellular matrix. Control experiments elucidate that a comparable extracellular matrix is not present in the interstitium of the matured kidney. Thus, generation of renal tubules in combination with advanced fixation of specimens for electron microscopy demonstrates that development of abnormal features in the newly developed interstitium has to be considered, when repair of renal parenchyma is performed by implantation of stem/progenitor cells.