Optimized fragmentation schemes and data analysis strategies for proteome-wide cross-link identification

Optimized fragmentation schemes and data analysis strategies for proteome-wide cross-link identification

Chemical cross-linking combined with mass spectrometry (XL-MS) can provide information on protein conformations and interactions in highly complex samples. Here the authors describe an improved XL-MS workflow to increase the depth and fidelity of cross-link identification using whole proteome databa...

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Bibliographic Details
Main Authors: Fan Liu, Philip Lössl, Richard Scheltema, Rosa Viner, Albert J. R. Heck
Format: Article
Language:English
Published: Nature Publishing Group 2017-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms15473
Description
Summary:Chemical cross-linking combined with mass spectrometry (XL-MS) can provide information on protein conformations and interactions in highly complex samples. Here the authors describe an improved XL-MS workflow to increase the depth and fidelity of cross-link identification using whole proteome databases.
ISSN:2041-1723

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