The effects of a magnetic IronQ complex on endothelial progenitor cells expansion in vitro
Introduction: In vitro proliferation and expansion of endothelial progenitor cells play important roles in the cell-based therapies for patient with acute myocardial infarction or ischemic heart disease. Objective: To determine the effect of magnetic IronQ complex on the proliferation of endothel...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Chaing Mai University
2016-01-01
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| Series: | Journal of Associated Medical Sciences |
| Subjects: | |
| Online Access: | https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/59925 |
| Summary: | Introduction: In vitro proliferation and expansion of endothelial progenitor cells play important roles in the cell-based therapies for patient with acute myocardial infarction or ischemic heart disease.
Objective: To determine the effect of magnetic IronQ complex on the proliferation of endothelial progenitor cells in vitro.
Methods: Peripheral blood mononuclear cells from venous blood was isolated by Ficoll-Hypaque gradient centrifugation method. PBMCs (1x106 cell/4-mL) were cultured with various concentrations of IronQ at 0, 100, 200, 300, 400 and 500 μg/mL for 17 days. Cell morphology was observed and recorded under inverted microscope. Specific marker for endothelial progenitor cells was characterized by immunostaining with CD34-FITC and analyzed by flow cytometer.
Results: Under inverted microscope, after 7 days of incubation, PBMCs showed stem cell-typical morphology surrounded by spindle-shaped cells. Interestingly, in the presence of IronQ, those colony-forming cells were increased in dose and time dependent manner. It was observed that only the cell incubated with IronQ was promoted spindle-shaped cells to form tube-like structure similar to the process of vasculogenesis. Cell incubated with IronQ for 10 days was positive with CD34 in dose dependent manner. Cells in the presence of IronQ at 500 μg/mL was strongly positive compared to others concentrations with the value of 47.66±2.52%. This indicated the increasing of endothelial progenitor cell proliferation.
Conclusion: This is the first study demonstrated the using of IronQ to promote endothelial progenitor cell proliferation in vitro without addition of any growth factors or specific growth activator leading to the cost reduction and also able to increase efficiency for cell-based therapy.
Bull Chiang Mai Assoc Med Sci 2016; 49(1): 106-113. Doi: 10.14456/jams.2016.3 |
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| ISSN: | 2539-6056 2539-6056 |