Combining Fluorescent Cell Sorting and Single B Cell Amplification to Screen the Monoclonal Antibody Gene against Human Glypican-1 in Pancreatic Cancer

Combining Fluorescent Cell Sorting and Single B Cell Amplification to Screen the Monoclonal Antibody Gene against Human Glypican-1 in Pancreatic Cancer

In this report, one novel method has been developed to screen the monoclonal antibody against human pancreatic cancer biomarker glypican-1 (GPC1) through the combination of fluorescent cell sorting and single B cell amplification. GPC1-positive B cells were sorted out from the peripheral blood monon...

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Bibliographic Details
Main Authors: Mi Huang, Yingying Ma, Xiaoyan Gao, Xinyang Li, Quan Ding, Chuxin Liu, Xiaopan Liu, Hang Zhang, Naibo Yang
Format: Article
Language:English
Published: Hindawi Limited 2021-01-01
Series:Journal of Oncology
Online Access:http://dx.doi.org/10.1155/2021/5646589

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