Manipulating and tracking single hepatocyte behavior during mouse liver regeneration by performing hydrodynamic tail vein injection
Summary: Studies to identify genes relevant to mammalian hepatocyte biology in vivo are largely carried out using germline genetic-engineering approaches, which can be costly and time-consuming. We describe hydrodynamic tail vein injection as an alternative approach to introduce genetic elements int...
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2021-06-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721001477 |
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doaj-7914a39995594cca840f1e37ca8e86862021-06-21T04:25:09ZengElsevierSTAR Protocols2666-16672021-06-0122100440Manipulating and tracking single hepatocyte behavior during mouse liver regeneration by performing hydrodynamic tail vein injectionShuang Wang0Marina Ruiz de Galarreta1Kirsten C. Sadler2Amaia Lujambio3Department of Medicine/Division of Liver Diseases, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Corresponding authorDepartment of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Liver Cancer Program, Division of Liver Diseases, Department of Medicine, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; The Precision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USAProgram in Biology, New York University Abu Dhabi, P.O. Box 129188, Abu Dhabi, United Arab EmiratesDepartment of Medicine/Division of Liver Diseases, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Liver Cancer Program, Division of Liver Diseases, Department of Medicine, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; The Precision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USASummary: Studies to identify genes relevant to mammalian hepatocyte biology in vivo are largely carried out using germline genetic-engineering approaches, which can be costly and time-consuming. We describe hydrodynamic tail vein injection as an alternative approach to introduce genetic elements into hepatocytes. Transfected hepatocytes can then be traced with a GFP reporter enabling the use of immunohistochemistry and FACS sorting to examine the changes in hepatocyte gene expression and proliferation during liver regeneration induced by 2/3 partial hepatectomy (PH).For complete details on the use and execution of this protocol, please refer to Wang et al. (2019).http://www.sciencedirect.com/science/article/pii/S2666166721001477Cell BiologyFlow Cytometry/Mass CytometryMetabolismMicroscopyModel OrganismsMolecular Biology |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Shuang Wang Marina Ruiz de Galarreta Kirsten C. Sadler Amaia Lujambio |
| spellingShingle |
Shuang Wang Marina Ruiz de Galarreta Kirsten C. Sadler Amaia Lujambio Manipulating and tracking single hepatocyte behavior during mouse liver regeneration by performing hydrodynamic tail vein injection STAR Protocols Cell Biology Flow Cytometry/Mass Cytometry Metabolism Microscopy Model Organisms Molecular Biology |
| author_facet |
Shuang Wang Marina Ruiz de Galarreta Kirsten C. Sadler Amaia Lujambio |
| author_sort |
Shuang Wang |
| title |
Manipulating and tracking single hepatocyte behavior during mouse liver regeneration by performing hydrodynamic tail vein injection |
| title_short |
Manipulating and tracking single hepatocyte behavior during mouse liver regeneration by performing hydrodynamic tail vein injection |
| title_full |
Manipulating and tracking single hepatocyte behavior during mouse liver regeneration by performing hydrodynamic tail vein injection |
| title_fullStr |
Manipulating and tracking single hepatocyte behavior during mouse liver regeneration by performing hydrodynamic tail vein injection |
| title_full_unstemmed |
Manipulating and tracking single hepatocyte behavior during mouse liver regeneration by performing hydrodynamic tail vein injection |
| title_sort |
manipulating and tracking single hepatocyte behavior during mouse liver regeneration by performing hydrodynamic tail vein injection |
| publisher |
Elsevier |
| series |
STAR Protocols |
| issn |
2666-1667 |
| publishDate |
2021-06-01 |
| description |
Summary: Studies to identify genes relevant to mammalian hepatocyte biology in vivo are largely carried out using germline genetic-engineering approaches, which can be costly and time-consuming. We describe hydrodynamic tail vein injection as an alternative approach to introduce genetic elements into hepatocytes. Transfected hepatocytes can then be traced with a GFP reporter enabling the use of immunohistochemistry and FACS sorting to examine the changes in hepatocyte gene expression and proliferation during liver regeneration induced by 2/3 partial hepatectomy (PH).For complete details on the use and execution of this protocol, please refer to Wang et al. (2019). |
| topic |
Cell Biology Flow Cytometry/Mass Cytometry Metabolism Microscopy Model Organisms Molecular Biology |
| url |
http://www.sciencedirect.com/science/article/pii/S2666166721001477 |
| work_keys_str_mv |
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