Csn3 gene is regulated by all-trans retinoic acid during neural differentiation in mouse P19 cells.

κ-Casein (CSN3) is known to play an essential role in controlling the stability of the milk micelles. We found that the expression of Csn3 was induced by all-trans retinoic acid (ATRA) during neural differentiation in P19 embryonal carcinoma cells from our study using DNA microarray. In this paper,...

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Main Authors: Rie Komori, Takanobu Kobayashi, Hikaru Matsuo, Katsuhito Kino, Hiroshi Miyazawa
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3629135?pdf=render
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spelling doaj-78cf5d35a2844b9f88cdaa887aa6beb42020-11-25T01:45:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0184e6193810.1371/journal.pone.0061938Csn3 gene is regulated by all-trans retinoic acid during neural differentiation in mouse P19 cells.Rie KomoriTakanobu KobayashiHikaru MatsuoKatsuhito KinoHiroshi Miyazawaκ-Casein (CSN3) is known to play an essential role in controlling the stability of the milk micelles. We found that the expression of Csn3 was induced by all-trans retinoic acid (ATRA) during neural differentiation in P19 embryonal carcinoma cells from our study using DNA microarray. In this paper, we describe the detailed time course of Csn3 expression and the induction mechanism of Csn3 transcription activation in this process. The Csn3 expression was induced rapidly and transiently within 24 h of ATRA treatment. Retinoic acid receptor (RAR)-specific agonists were used in expression analysis to identify the RAR subtype involved upregulation of Csn3; a RARα-specific agonist mimicked the effects of ATRA on induction of Csn3 expression. Therefore, RARα may be the RAR subtype mediating the effects of ATRA on the induction of Csn3 gene transcription in this differentiation-promoting process of P19 cells. We found that the promoter region of Csn3 contained a typical consensus retinoic acid response element (RARE), and this RARE was necessary for ATRA-dependent transcriptional regulation. We confirmed that RARα bound to this RARE sequence in P19 cells. These findings indicated that the Csn3 expression is upregulated via ATRA-bound RARα and binding of this receptor to the RARE in the Csn3 promoter region. This will certainly serve as a first step forward unraveling the mysteries of induction of Csn3 in the process of neural differentiation.http://europepmc.org/articles/PMC3629135?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Rie Komori
Takanobu Kobayashi
Hikaru Matsuo
Katsuhito Kino
Hiroshi Miyazawa
spellingShingle Rie Komori
Takanobu Kobayashi
Hikaru Matsuo
Katsuhito Kino
Hiroshi Miyazawa
Csn3 gene is regulated by all-trans retinoic acid during neural differentiation in mouse P19 cells.
PLoS ONE
author_facet Rie Komori
Takanobu Kobayashi
Hikaru Matsuo
Katsuhito Kino
Hiroshi Miyazawa
author_sort Rie Komori
title Csn3 gene is regulated by all-trans retinoic acid during neural differentiation in mouse P19 cells.
title_short Csn3 gene is regulated by all-trans retinoic acid during neural differentiation in mouse P19 cells.
title_full Csn3 gene is regulated by all-trans retinoic acid during neural differentiation in mouse P19 cells.
title_fullStr Csn3 gene is regulated by all-trans retinoic acid during neural differentiation in mouse P19 cells.
title_full_unstemmed Csn3 gene is regulated by all-trans retinoic acid during neural differentiation in mouse P19 cells.
title_sort csn3 gene is regulated by all-trans retinoic acid during neural differentiation in mouse p19 cells.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2013-01-01
description κ-Casein (CSN3) is known to play an essential role in controlling the stability of the milk micelles. We found that the expression of Csn3 was induced by all-trans retinoic acid (ATRA) during neural differentiation in P19 embryonal carcinoma cells from our study using DNA microarray. In this paper, we describe the detailed time course of Csn3 expression and the induction mechanism of Csn3 transcription activation in this process. The Csn3 expression was induced rapidly and transiently within 24 h of ATRA treatment. Retinoic acid receptor (RAR)-specific agonists were used in expression analysis to identify the RAR subtype involved upregulation of Csn3; a RARα-specific agonist mimicked the effects of ATRA on induction of Csn3 expression. Therefore, RARα may be the RAR subtype mediating the effects of ATRA on the induction of Csn3 gene transcription in this differentiation-promoting process of P19 cells. We found that the promoter region of Csn3 contained a typical consensus retinoic acid response element (RARE), and this RARE was necessary for ATRA-dependent transcriptional regulation. We confirmed that RARα bound to this RARE sequence in P19 cells. These findings indicated that the Csn3 expression is upregulated via ATRA-bound RARα and binding of this receptor to the RARE in the Csn3 promoter region. This will certainly serve as a first step forward unraveling the mysteries of induction of Csn3 in the process of neural differentiation.
url http://europepmc.org/articles/PMC3629135?pdf=render
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