Efficient Generation of CRISPR/Cas9-Mediated Homozygous/Biallelic Medicago truncatula Mutants Using a Hairy Root System

Efficient Generation of CRISPR/Cas9-Mediated Homozygous/Biallelic Medicago truncatula Mutants Using a Hairy Root System

In the process of acquiring mutants mediated by CRISPR/Cas9, plantlets are often regenerated from both mutated and non-mutated cells in a random manner, which increase the odds of chimeric mutated plant. In general, it’s necessary to infect more explants or grow to next generation for the need of ge...

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Bibliographic Details
Main Authors: Hailing Zhang, Yingping Cao, Huan Zhang, Yue Xu, Chuanen Zhou, Wenwen Liu, Ruifen Zhu, Chen Shang, Jikai Li, Zhongbao Shen, Siyi Guo, Zhubing Hu, Chunxiang Fu, Dequan Sun
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-03-01
Series:Frontiers in Plant Science
Subjects:
CRISPR/Cas9
genome editing
hairy root
homozygous/biallelic mutant
Medicago truncatula
Online Access:https://www.frontiersin.org/article/10.3389/fpls.2020.00294/full

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