Therapeutic benefit of resveratrol in 5-fluorouracil-induced nephrotoxicity in rats
Background: The prevention of nephrotoxicity caused by 5-fluorouracil (5-FU) can improve patients' adherence to treatment.Aim and Objective: This study assessed the ability of resveratrol (RES) to prevent 5-FU-induced nephrotoxicity in rats. Materials and Methods: Forty adult male albino rats r...
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doaj-78754f98c7824d6e94ec9c3299048cfb2020-12-02T12:27:30ZengWolters Kluwer Medknow PublicationsBiomedical Research Journal2349-36662349-36742019-01-0162727710.4103/BMRJ.BMRJ_19_19Therapeutic benefit of resveratrol in 5-fluorouracil-induced nephrotoxicity in ratsElias AdikwuInnocent BiradeeTemitope Omolade OgungbaikeBackground: The prevention of nephrotoxicity caused by 5-fluorouracil (5-FU) can improve patients' adherence to treatment.Aim and Objective: This study assessed the ability of resveratrol (RES) to prevent 5-FU-induced nephrotoxicity in rats. Materials and Methods: Forty adult male albino rats randomized into eight groups of n = 5 were used. Group A (control) was administered with 0.2 mL of normal saline intraperitoneally (i.p.), whereas Groups B-D were administered with 10, 20, and 40 mg/kg of RES daily for 5 days respectively. Group E was administered with 20 mg/kg of 5-FU ip daily for 5 days. Groups F-H were administered with 10 mg/kg of RES + 20 mg/kg of 5-FU, 20 mg/kg of RES + 20 mg/kg of 5-FU, and 40 mg/kg of RES + 20 mg/kg of 5-FU ip daily for 5 days, respectively. Blood samples were collected after rats were sacrificed and evaluated for serum renal function biomarkers. Kidneys were evaluated for oxidative stress markers and histology. Results: Serum creatinine, urea, and uric acid levels were significantly (P < 0.001) increased, whereas total protein, albumin, potassium, sodium, chloride, and bicarbonate levels were significantly (P < 0.001) decreased in 5-FU-treated rats when compared to control. Kidney superoxide dismutase, glutathione, glutathione peroxidase, and catalase levels were significantly (P < 0.001) decreased, whereas malondialdehyde levels were significantly increased in 5-FU-treated rats when compared to control. Furthermore, the kidneys of 5-FU-treated rats showed tubular necroses and atrophic glomeruli. The aforementioned nephrotoxic changes were significantly abrogated in rats supplemented with 10 mg/kg (P < 0.05), 20 mg/kg (P < 0.01), and 40 mg/kg (P < 0.001) of RES when compared to 5-FU. Conclusion: RES may have therapeutic benefit in nephrotoxicity caused by 5-FU.http://www.brjnmims.org/article.asp?issn=2349-3666;year=2019;volume=6;issue=2;spage=72;epage=77;aulast=Adikwu5-fluorourcilantioxidantkidneyrattoxicity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elias Adikwu Innocent Biradee Temitope Omolade Ogungbaike |
spellingShingle |
Elias Adikwu Innocent Biradee Temitope Omolade Ogungbaike Therapeutic benefit of resveratrol in 5-fluorouracil-induced nephrotoxicity in rats Biomedical Research Journal 5-fluorourcil antioxidant kidney rat toxicity |
author_facet |
Elias Adikwu Innocent Biradee Temitope Omolade Ogungbaike |
author_sort |
Elias Adikwu |
title |
Therapeutic benefit of resveratrol in 5-fluorouracil-induced nephrotoxicity in rats |
title_short |
Therapeutic benefit of resveratrol in 5-fluorouracil-induced nephrotoxicity in rats |
title_full |
Therapeutic benefit of resveratrol in 5-fluorouracil-induced nephrotoxicity in rats |
title_fullStr |
Therapeutic benefit of resveratrol in 5-fluorouracil-induced nephrotoxicity in rats |
title_full_unstemmed |
Therapeutic benefit of resveratrol in 5-fluorouracil-induced nephrotoxicity in rats |
title_sort |
therapeutic benefit of resveratrol in 5-fluorouracil-induced nephrotoxicity in rats |
publisher |
Wolters Kluwer Medknow Publications |
series |
Biomedical Research Journal |
issn |
2349-3666 2349-3674 |
publishDate |
2019-01-01 |
description |
Background: The prevention of nephrotoxicity caused by 5-fluorouracil (5-FU) can improve patients' adherence to treatment.Aim and Objective: This study assessed the ability of resveratrol (RES) to prevent 5-FU-induced nephrotoxicity in rats. Materials and Methods: Forty adult male albino rats randomized into eight groups of n = 5 were used. Group A (control) was administered with 0.2 mL of normal saline intraperitoneally (i.p.), whereas Groups B-D were administered with 10, 20, and 40 mg/kg of RES daily for 5 days respectively. Group E was administered with 20 mg/kg of 5-FU ip daily for 5 days. Groups F-H were administered with 10 mg/kg of RES + 20 mg/kg of 5-FU, 20 mg/kg of RES + 20 mg/kg of 5-FU, and 40 mg/kg of RES + 20 mg/kg of 5-FU ip daily for 5 days, respectively. Blood samples were collected after rats were sacrificed and evaluated for serum renal function biomarkers. Kidneys were evaluated for oxidative stress markers and histology. Results: Serum creatinine, urea, and uric acid levels were significantly (P < 0.001) increased, whereas total protein, albumin, potassium, sodium, chloride, and bicarbonate levels were significantly (P < 0.001) decreased in 5-FU-treated rats when compared to control. Kidney superoxide dismutase, glutathione, glutathione peroxidase, and catalase levels were significantly (P < 0.001) decreased, whereas malondialdehyde levels were significantly increased in 5-FU-treated rats when compared to control. Furthermore, the kidneys of 5-FU-treated rats showed tubular necroses and atrophic glomeruli. The aforementioned nephrotoxic changes were significantly abrogated in rats supplemented with 10 mg/kg (P < 0.05), 20 mg/kg (P < 0.01), and 40 mg/kg (P < 0.001) of RES when compared to 5-FU. Conclusion: RES may have therapeutic benefit in nephrotoxicity caused by 5-FU. |
topic |
5-fluorourcil antioxidant kidney rat toxicity |
url |
http://www.brjnmims.org/article.asp?issn=2349-3666;year=2019;volume=6;issue=2;spage=72;epage=77;aulast=Adikwu |
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