Therapeutic benefit of resveratrol in 5-fluorouracil-induced nephrotoxicity in rats

Background: The prevention of nephrotoxicity caused by 5-fluorouracil (5-FU) can improve patients' adherence to treatment.Aim and Objective: This study assessed the ability of resveratrol (RES) to prevent 5-FU-induced nephrotoxicity in rats. Materials and Methods: Forty adult male albino rats r...

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Main Authors: Elias Adikwu, Innocent Biradee, Temitope Omolade Ogungbaike
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2019-01-01
Series:Biomedical Research Journal
Subjects:
rat
Online Access:http://www.brjnmims.org/article.asp?issn=2349-3666;year=2019;volume=6;issue=2;spage=72;epage=77;aulast=Adikwu
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spelling doaj-78754f98c7824d6e94ec9c3299048cfb2020-12-02T12:27:30ZengWolters Kluwer Medknow PublicationsBiomedical Research Journal2349-36662349-36742019-01-0162727710.4103/BMRJ.BMRJ_19_19Therapeutic benefit of resveratrol in 5-fluorouracil-induced nephrotoxicity in ratsElias AdikwuInnocent BiradeeTemitope Omolade OgungbaikeBackground: The prevention of nephrotoxicity caused by 5-fluorouracil (5-FU) can improve patients' adherence to treatment.Aim and Objective: This study assessed the ability of resveratrol (RES) to prevent 5-FU-induced nephrotoxicity in rats. Materials and Methods: Forty adult male albino rats randomized into eight groups of n = 5 were used. Group A (control) was administered with 0.2 mL of normal saline intraperitoneally (i.p.), whereas Groups B-D were administered with 10, 20, and 40 mg/kg of RES daily for 5 days respectively. Group E was administered with 20 mg/kg of 5-FU ip daily for 5 days. Groups F-H were administered with 10 mg/kg of RES + 20 mg/kg of 5-FU, 20 mg/kg of RES + 20 mg/kg of 5-FU, and 40 mg/kg of RES + 20 mg/kg of 5-FU ip daily for 5 days, respectively. Blood samples were collected after rats were sacrificed and evaluated for serum renal function biomarkers. Kidneys were evaluated for oxidative stress markers and histology. Results: Serum creatinine, urea, and uric acid levels were significantly (P < 0.001) increased, whereas total protein, albumin, potassium, sodium, chloride, and bicarbonate levels were significantly (P < 0.001) decreased in 5-FU-treated rats when compared to control. Kidney superoxide dismutase, glutathione, glutathione peroxidase, and catalase levels were significantly (P < 0.001) decreased, whereas malondialdehyde levels were significantly increased in 5-FU-treated rats when compared to control. Furthermore, the kidneys of 5-FU-treated rats showed tubular necroses and atrophic glomeruli. The aforementioned nephrotoxic changes were significantly abrogated in rats supplemented with 10 mg/kg (P < 0.05), 20 mg/kg (P < 0.01), and 40 mg/kg (P < 0.001) of RES when compared to 5-FU. Conclusion: RES may have therapeutic benefit in nephrotoxicity caused by 5-FU.http://www.brjnmims.org/article.asp?issn=2349-3666;year=2019;volume=6;issue=2;spage=72;epage=77;aulast=Adikwu5-fluorourcilantioxidantkidneyrattoxicity
collection DOAJ
language English
format Article
sources DOAJ
author Elias Adikwu
Innocent Biradee
Temitope Omolade Ogungbaike
spellingShingle Elias Adikwu
Innocent Biradee
Temitope Omolade Ogungbaike
Therapeutic benefit of resveratrol in 5-fluorouracil-induced nephrotoxicity in rats
Biomedical Research Journal
5-fluorourcil
antioxidant
kidney
rat
toxicity
author_facet Elias Adikwu
Innocent Biradee
Temitope Omolade Ogungbaike
author_sort Elias Adikwu
title Therapeutic benefit of resveratrol in 5-fluorouracil-induced nephrotoxicity in rats
title_short Therapeutic benefit of resveratrol in 5-fluorouracil-induced nephrotoxicity in rats
title_full Therapeutic benefit of resveratrol in 5-fluorouracil-induced nephrotoxicity in rats
title_fullStr Therapeutic benefit of resveratrol in 5-fluorouracil-induced nephrotoxicity in rats
title_full_unstemmed Therapeutic benefit of resveratrol in 5-fluorouracil-induced nephrotoxicity in rats
title_sort therapeutic benefit of resveratrol in 5-fluorouracil-induced nephrotoxicity in rats
publisher Wolters Kluwer Medknow Publications
series Biomedical Research Journal
issn 2349-3666
2349-3674
publishDate 2019-01-01
description Background: The prevention of nephrotoxicity caused by 5-fluorouracil (5-FU) can improve patients' adherence to treatment.Aim and Objective: This study assessed the ability of resveratrol (RES) to prevent 5-FU-induced nephrotoxicity in rats. Materials and Methods: Forty adult male albino rats randomized into eight groups of n = 5 were used. Group A (control) was administered with 0.2 mL of normal saline intraperitoneally (i.p.), whereas Groups B-D were administered with 10, 20, and 40 mg/kg of RES daily for 5 days respectively. Group E was administered with 20 mg/kg of 5-FU ip daily for 5 days. Groups F-H were administered with 10 mg/kg of RES + 20 mg/kg of 5-FU, 20 mg/kg of RES + 20 mg/kg of 5-FU, and 40 mg/kg of RES + 20 mg/kg of 5-FU ip daily for 5 days, respectively. Blood samples were collected after rats were sacrificed and evaluated for serum renal function biomarkers. Kidneys were evaluated for oxidative stress markers and histology. Results: Serum creatinine, urea, and uric acid levels were significantly (P < 0.001) increased, whereas total protein, albumin, potassium, sodium, chloride, and bicarbonate levels were significantly (P < 0.001) decreased in 5-FU-treated rats when compared to control. Kidney superoxide dismutase, glutathione, glutathione peroxidase, and catalase levels were significantly (P < 0.001) decreased, whereas malondialdehyde levels were significantly increased in 5-FU-treated rats when compared to control. Furthermore, the kidneys of 5-FU-treated rats showed tubular necroses and atrophic glomeruli. The aforementioned nephrotoxic changes were significantly abrogated in rats supplemented with 10 mg/kg (P < 0.05), 20 mg/kg (P < 0.01), and 40 mg/kg (P < 0.001) of RES when compared to 5-FU. Conclusion: RES may have therapeutic benefit in nephrotoxicity caused by 5-FU.
topic 5-fluorourcil
antioxidant
kidney
rat
toxicity
url http://www.brjnmims.org/article.asp?issn=2349-3666;year=2019;volume=6;issue=2;spage=72;epage=77;aulast=Adikwu
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AT innocentbiradee therapeuticbenefitofresveratrolin5fluorouracilinducednephrotoxicityinrats
AT temitopeomoladeogungbaike therapeuticbenefitofresveratrolin5fluorouracilinducednephrotoxicityinrats
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