Discrimination of Radix Polygoni Multiflori from different geographical areas by UPLC-QTOF/MS combined with chemometrics

• Main Authors: Jin-Fa Tang, Wei-Xia Li, Fan Zhang, Yu-Hui Li, Ying-Jie Cao, Ya Zhao, Xue-Lin Li, Zhi-Jie Ma
• Format: Article
• Language: English
• Published: BMC 2017-12-01
• Series: Chinese Medicine
• Subjects: UPLC-QTOF/MS; Radix Polygoni Multiflori; Different geographical origins; Chemical markers; Partial least squared discriminant analysis
• Online Access: http://link.springer.com/article/10.1186/s13020-017-0155-8

Abstract Background Nowadays, Radix Polygoni Multiflori (RPM, Heshouwu in Chinese) from different geographical origins were used in clinic. In order to characterize the chemical profiles of different geographical origins of RPM samples, ultra-high performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF/MS) combined with chemometrics (partial least squared discriminant analysis, PLS‑DA) method was applied in the present study. Methods The chromatography, chemical composition and MS information of RPM samples from 18 geographical origins were acquired and profiled by UPLC-QTOF/MS. The chemical markers contributing the differentiation of RPM samples were observed and characterized by supervised PLS‑DA method of chemometrics. Results The chemical composition differences of RPM samples derived from 18 different geographical origins were observed. Nine chemical markers were tentatively identified which could be used as specific chemical markers for the differentiation of geographical RPM samples. Conclusions UPLC-QTOF/MS method coupled with chemometrics analysis has potential to be used for discriminating different geographical TCMs. Results will help to develop strategies for conservation and utilization of RPM samples.