Influence of prior influenza vaccination on antibody and B-cell responses.

Currently two vaccines, trivalent inactivated influenza vaccine (TIV) and live attenuated influenza vaccine (LAIV), are licensed in the USA. Despite previous studies on immune responses induced by these two vaccines, a comparative study of the influence of prior influenza vaccination on serum antibo...

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Main Authors: Sanae Sasaki, Xiao-Song He, Tyson H Holmes, Cornelia L Dekker, George W Kemble, Ann M Arvin, Harry B Greenberg
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2008-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2500171?pdf=render
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spelling doaj-78462b7fa8b743ce8faad3e12986d4302020-11-25T02:26:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-0138e297510.1371/journal.pone.0002975Influence of prior influenza vaccination on antibody and B-cell responses.Sanae SasakiXiao-Song HeTyson H HolmesCornelia L DekkerGeorge W KembleAnn M ArvinHarry B GreenbergCurrently two vaccines, trivalent inactivated influenza vaccine (TIV) and live attenuated influenza vaccine (LAIV), are licensed in the USA. Despite previous studies on immune responses induced by these two vaccines, a comparative study of the influence of prior influenza vaccination on serum antibody and B-cell responses to new LAIV or TIV vaccination has not been reported. During the 2005/6 influenza season, we quantified the serum antibody and B-cell responses to LAIV or TIV in adults with differing influenza vaccination histories in the prior year: LAIV, TIV, or neither. Blood samples were collected on days 0, 7-9 and 21-35 after immunization and used for serum HAI assay and B-cell assays. Total and influenza-specific circulating IgG and IgA antibody secreting cells (ASC) in PBMC were detected by direct ELISPOT assay. Memory B cells were also tested by ELISPOT after polyclonal stimulation of PBMC in vitro. Serum antibody, effector, and memory B-cell responses were greater in TIV recipients than LAIV recipients. Prior year TIV recipients had significantly higher baseline HAI titers, but lower HAI response after vaccination with either TIV or LAIV, and lower IgA ASC response after vaccination with TIV than prior year LAIV or no vaccination recipients. Lower levels of baseline HAI titer were associated with a greater fold-increase of HAI titer and ASC number after vaccination, which also differed by type of vaccine. Our findings suggest that the type of vaccine received in the prior year affects the serum antibody and the B-cell responses to subsequent vaccination. In particular, prior year TIV vaccination is associated with sustained higher HAI titer one year later but lower antibody response to new LAIV or TIV vaccination, and a lower effector B-cell response to new TIV but not LAIV vaccination.http://europepmc.org/articles/PMC2500171?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Sanae Sasaki
Xiao-Song He
Tyson H Holmes
Cornelia L Dekker
George W Kemble
Ann M Arvin
Harry B Greenberg
spellingShingle Sanae Sasaki
Xiao-Song He
Tyson H Holmes
Cornelia L Dekker
George W Kemble
Ann M Arvin
Harry B Greenberg
Influence of prior influenza vaccination on antibody and B-cell responses.
PLoS ONE
author_facet Sanae Sasaki
Xiao-Song He
Tyson H Holmes
Cornelia L Dekker
George W Kemble
Ann M Arvin
Harry B Greenberg
author_sort Sanae Sasaki
title Influence of prior influenza vaccination on antibody and B-cell responses.
title_short Influence of prior influenza vaccination on antibody and B-cell responses.
title_full Influence of prior influenza vaccination on antibody and B-cell responses.
title_fullStr Influence of prior influenza vaccination on antibody and B-cell responses.
title_full_unstemmed Influence of prior influenza vaccination on antibody and B-cell responses.
title_sort influence of prior influenza vaccination on antibody and b-cell responses.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2008-01-01
description Currently two vaccines, trivalent inactivated influenza vaccine (TIV) and live attenuated influenza vaccine (LAIV), are licensed in the USA. Despite previous studies on immune responses induced by these two vaccines, a comparative study of the influence of prior influenza vaccination on serum antibody and B-cell responses to new LAIV or TIV vaccination has not been reported. During the 2005/6 influenza season, we quantified the serum antibody and B-cell responses to LAIV or TIV in adults with differing influenza vaccination histories in the prior year: LAIV, TIV, or neither. Blood samples were collected on days 0, 7-9 and 21-35 after immunization and used for serum HAI assay and B-cell assays. Total and influenza-specific circulating IgG and IgA antibody secreting cells (ASC) in PBMC were detected by direct ELISPOT assay. Memory B cells were also tested by ELISPOT after polyclonal stimulation of PBMC in vitro. Serum antibody, effector, and memory B-cell responses were greater in TIV recipients than LAIV recipients. Prior year TIV recipients had significantly higher baseline HAI titers, but lower HAI response after vaccination with either TIV or LAIV, and lower IgA ASC response after vaccination with TIV than prior year LAIV or no vaccination recipients. Lower levels of baseline HAI titer were associated with a greater fold-increase of HAI titer and ASC number after vaccination, which also differed by type of vaccine. Our findings suggest that the type of vaccine received in the prior year affects the serum antibody and the B-cell responses to subsequent vaccination. In particular, prior year TIV vaccination is associated with sustained higher HAI titer one year later but lower antibody response to new LAIV or TIV vaccination, and a lower effector B-cell response to new TIV but not LAIV vaccination.
url http://europepmc.org/articles/PMC2500171?pdf=render
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