Comparison of two elution buffers used in an adsorption-elution method with a negatively charged membrane to recover Norovirus from lettuce (Lactuca sativa)

Comparison of two elution buffers used in an adsorption-elution method with a negatively charged membrane to recover Norovirus from lettuce (Lactuca sativa)

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Phosphate saline buffer (PBS) and glycine buffer (GB) were evaluated as elution buffers in an adsorption-elution method using a negatively charged membrane associated with quantitative polymerase chain reaction (qPCR) and seminested PCR for detection of Norovirus genogroup II (NoV GII) from lettuce...

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Bibliographic Details
Main Authors: Marcelo Luiz Lima Brandão, Davi de Oliveira Almeida, Victor Augustus Marin, Marize Pereira Miagostovich
Format: Article
Language:English
Published: Fundação Oswaldo Cruz (FIOCRUZ) 2014-08-01
Series:Vigilância Sanitária em Debate: Sociedade, Ciência & Tecnologia
Subjects:
Norovirus
Filtration
Lettuce
PP7 Bacteriophage
Internal Control Process
Online Access:https://hm-visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/195
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