Comparison of two elution buffers used in an adsorption-elution method with a negatively charged membrane to recover Norovirus from lettuce (Lactuca sativa)
Phosphate saline buffer (PBS) and glycine buffer (GB) were evaluated as elution buffers in an adsorption-elution method using a negatively charged membrane associated with quantitative polymerase chain reaction (qPCR) and seminested PCR for detection of Norovirus genogroup II (NoV GII) from lettuce...
Main Authors: | Marcelo Luiz Lima Brandão, Davi de Oliveira Almeida, Victor Augustus Marin, Marize Pereira Miagostovich |
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Format: | Article |
Language: | English |
Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2014-08-01
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Series: | Vigilância Sanitária em Debate: Sociedade, Ciência & Tecnologia |
Subjects: | |
Online Access: | https://hm-visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/195 |
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