Study of Uricase Properties Partially Purified from Pisum sativum Seeds and its Effect on Uric Acid Level in Mice

• Main Author: Israa Sahl Ahmad Al.flayeh
• Format: Article
• Language: Arabic
• Published: College of Education for Pure Sciences 2013-10-01
• Series: مجلة التربية والعلم
• Subjects: uricase properties; pisum sativum seeds; uric acid; mice
• Online Access: https://edusj.mosuljournals.com/article_162848_aad3c23a0431b26efb7a35e04e54b3a9.pdf

<strong>Abstract</strong><br /> Uricase (urate oxidase) was partially purified from <em>Pisum sativum</em> seeds by ammonium sulfate precipitation ,dialysis and anion exchange  chromatography techniques. One proteinous peak was obtained containing two isomers of uricase I and II with specific activity  (5.92×10^-3and 4.9955×10^-3) unit/mg protein and with purification fold(31.32and 26.43) respectively compared to crude enzyme.<br /> The optimum conditions for the purified enzyme was determined using uric acid as a substrate. The results showed that maximum activity with glycine-NaOH buffer at pH=7, 40°C, substrate concentration 100mM Vmax=6.25×10^-2 U/mg protein and the Km=50mM, 100µl enzyme volume and the reaction velocity was increased with copper ion.<br /> The active dose of uricase for reducing uric acid was determined in healthy mice 400µg/kg body weight, and the best treatment was obtained by the injection of enzyme with allopurinal intraperitonally.