Phytochemical Profile and Antioxidant Capacity of Coffee Plant Organs Compared to Green and Roasted Coffee Beans

• Main Authors: Robert Acidri, Yumiko Sawai, Yuko Sugimoto, Takuo Handa, Daisuke Sasagawa, Tsugiyaki Masunaga, Sadahiro Yamamoto, Eiji Nishihara
• Format: Article
• Language: English
• Published: MDPI AG 2020-01-01
• Series: Antioxidants
• Subjects: alkaloids; antioxidant capacity; carotenoids; chlorophylls; <i>coffea arabica</i> l.; phenolic compounds; sucrose
• Online Access: https://www.mdpi.com/2076-3921/9/2/93

The current study investigates the phytochemical composition of coffee plant organs and their corresponding antioxidant capacities compared to green and roasted coffee beans. HPLC analysis indicated that the investigated compounds were present in all organs except mangiferin, which was absent in roots, stems and seeds, and caffeine, which was absent in stems and roots. Total phytochemicals were highest in the green beans (GB) at 9.70 mg g^&#8722;1 dry weight (DW), while roasting caused a 66% decline in the roasted beans (RB). This decline resulted more from 5&#8722;CQA and sucrose decomposition by 68% and 97%, respectively, while caffeine and trigonelline were not significantly thermally affected. Roasting increased the total phenolic content (TPC) by 20.8% which was associated with an increase of 68.8%, 47.5% and 13.4% in the antioxidant capacity (TEAC) determined by 2,2&#8722;diphenyl&#8722;1&#8722;picryl hydrazyl radical (DPPH), 2,2&#8722;azino bis (3&#8722;ethyl benzothiazoline&#8722;6&#8722;sulphonic acid) radical (ABTS) and Ferric ion reducing antioxidant power (FRAP) assays, respectively. Amongst the leaves, the youngest (L1) contained the highest content at 8.23 mg g^&#8722;1 DW, which gradually reduced with leaf age to 5.57 mg g^&#8722;1 DW in the oldest (L6). Leaves also contained the highest TPC (over 60 mg g^&#8722;1 GAE) and exhibited high TEAC, the latter being highest in L1 at 328.0, 345.7 and 1097.4, and least in L6 at 304.6, 294.5 and 755.1 &#181;mol Trolox g^&#8722;1 sample for the respective assays. Phytochemical accumulation, TPC and TEAC were least in woody stem (WS) at 1.42 mg g^&#8722;1 DW; 8.7 mg g^&#8722;1 GAE; 21.9, 24.9 and 110.0 &#181;mol Trolox g^&#8722;1 sample; while herbaceous stem (HS) contained up to 4.37 mg g^&#8722;1 DW; 27.8 mg g^&#8722;1 GAE; 110.9, 124.8 and 469.7 &#181;mol Trolox g^&#8722;1 sample, respectively. Roots contained up to 1.85 mg g^&#8722;1 DW, 15.8 mg^&#8722;1 GAE and TEAC of 36.8, 41.5 and 156.7 &#181;mol Trolox g^&#8722;1 sample. Amongst the organs, therefore, coffee leaves possessed higher values than roasted beans on the basis of phytochemicals, TPC and TEAC. Leaves also contain carotenoids and chlorophylls pigments with potent health benefits. With appropriate processing methods, a beverage prepared from leaves (coffee leaf tea) could be a rich source of phytochemicals and antioxidants with therapeutic and pharmacological values for human health.