| Summary: | In this study, a SPAD value detection system was developed based on a 25-wavelength spectral sensor to give a real-time indication of the nutrition distribution of potato plants in the field. Two major advantages of the detection system include the automatic segmentation of spectral images and the real-time detection of SPAD value, a recommended indicating parameter of chlorophyll content. The modified difference vegetation index (MDVI) linking the Otsu algorithm (OTSU) and the connected domain-labeling (CDL) method (MDVI–OTSU–CDL) is proposed to accurately extract the potato plant. Additionally, the segmentation accuracy under different modified coefficients of MDVI was analyzed. Then, the reflectance of potato plants was extracted by the segmented mask images. The partial least squares (PLS) regression was employed to establish the SPAD value detection model based on sensitive variables selected using the uninformative variable elimination (UVE) algorithm. Based on the segmented spectral image and the UVE–PLS model, the visualization distribution map of SPAD value was drawn by pseudo-color processing technology. Finally, the testing dataset was employed to measure the stability and practicality of the developed detection system. This study provides a powerful support for the real-time detection of SPAD value and the distribution of crops in the field.
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