Molecular Characterization of a Novel Ourmia-Like Virus Infecting <i>Phoma matteucciicola</i>
Here, we report a novel (+) ssRNA mycovirus, Phoma matteucciicola ourmia-like virus 1 (PmOLV1), isolated from <i>Phoma matteucciicola</i> strain LG915-1. The genome of PmOLV1 was 2603 nucleotides long and contained a single open reading frame (ORF), which could be translated into a produ...
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MDPI AG
2020-02-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/2/231 |
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doaj-77374a31f3614f778a2f86907b762cf72020-11-25T02:39:14ZengMDPI AGViruses1999-49152020-02-0112223110.3390/v12020231v12020231Molecular Characterization of a Novel Ourmia-Like Virus Infecting <i>Phoma matteucciicola</i>Jia Zhou0Yuhua Wang1Xiaofei Liang2Changping Xie3Wenbo Liu4Weiguo Miao5Zhensheng Kang6Li Zheng7Key Laboratory of Green Prevention and Control of Tropical Plant Disease and Pests, Ministry of Education and College of Plant Protection, Hainan University, Haikou 570228, ChinaKey Laboratory of Green Prevention and Control of Tropical Plant Disease and Pests, Ministry of Education and College of Plant Protection, Hainan University, Haikou 570228, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Green Prevention and Control of Tropical Plant Disease and Pests, Ministry of Education and College of Plant Protection, Hainan University, Haikou 570228, ChinaKey Laboratory of Green Prevention and Control of Tropical Plant Disease and Pests, Ministry of Education and College of Plant Protection, Hainan University, Haikou 570228, ChinaKey Laboratory of Green Prevention and Control of Tropical Plant Disease and Pests, Ministry of Education and College of Plant Protection, Hainan University, Haikou 570228, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Green Prevention and Control of Tropical Plant Disease and Pests, Ministry of Education and College of Plant Protection, Hainan University, Haikou 570228, ChinaHere, we report a novel (+) ssRNA mycovirus, Phoma matteucciicola ourmia-like virus 1 (PmOLV1), isolated from <i>Phoma matteucciicola</i> strain LG915-1. The genome of PmOLV1 was 2603 nucleotides long and contained a single open reading frame (ORF), which could be translated into a product of RNA-dependent RNA polymerase (RdRp) by both standard and mitochondrial genetic codons. Cellular fractionation assay indicated that PmOLV1 RNAs are likely more enriched in mitochondria than in cytoplasm. Phylogenetic analysis indicated that PmOLV1 is a new member of the genus <i>Penoulivirus</i> (recently proposed) within the family <i>Botourmiaviridae</i>.https://www.mdpi.com/1999-4915/12/2/231<i>phoma matteucciicola</i>mycovirusourmiavirus<i>narnaviridae</i> |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Jia Zhou Yuhua Wang Xiaofei Liang Changping Xie Wenbo Liu Weiguo Miao Zhensheng Kang Li Zheng |
| spellingShingle |
Jia Zhou Yuhua Wang Xiaofei Liang Changping Xie Wenbo Liu Weiguo Miao Zhensheng Kang Li Zheng Molecular Characterization of a Novel Ourmia-Like Virus Infecting <i>Phoma matteucciicola</i> Viruses <i>phoma matteucciicola</i> mycovirus ourmiavirus <i>narnaviridae</i> |
| author_facet |
Jia Zhou Yuhua Wang Xiaofei Liang Changping Xie Wenbo Liu Weiguo Miao Zhensheng Kang Li Zheng |
| author_sort |
Jia Zhou |
| title |
Molecular Characterization of a Novel Ourmia-Like Virus Infecting <i>Phoma matteucciicola</i> |
| title_short |
Molecular Characterization of a Novel Ourmia-Like Virus Infecting <i>Phoma matteucciicola</i> |
| title_full |
Molecular Characterization of a Novel Ourmia-Like Virus Infecting <i>Phoma matteucciicola</i> |
| title_fullStr |
Molecular Characterization of a Novel Ourmia-Like Virus Infecting <i>Phoma matteucciicola</i> |
| title_full_unstemmed |
Molecular Characterization of a Novel Ourmia-Like Virus Infecting <i>Phoma matteucciicola</i> |
| title_sort |
molecular characterization of a novel ourmia-like virus infecting <i>phoma matteucciicola</i> |
| publisher |
MDPI AG |
| series |
Viruses |
| issn |
1999-4915 |
| publishDate |
2020-02-01 |
| description |
Here, we report a novel (+) ssRNA mycovirus, Phoma matteucciicola ourmia-like virus 1 (PmOLV1), isolated from <i>Phoma matteucciicola</i> strain LG915-1. The genome of PmOLV1 was 2603 nucleotides long and contained a single open reading frame (ORF), which could be translated into a product of RNA-dependent RNA polymerase (RdRp) by both standard and mitochondrial genetic codons. Cellular fractionation assay indicated that PmOLV1 RNAs are likely more enriched in mitochondria than in cytoplasm. Phylogenetic analysis indicated that PmOLV1 is a new member of the genus <i>Penoulivirus</i> (recently proposed) within the family <i>Botourmiaviridae</i>. |
| topic |
<i>phoma matteucciicola</i> mycovirus ourmiavirus <i>narnaviridae</i> |
| url |
https://www.mdpi.com/1999-4915/12/2/231 |
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