Semi-preparative isolation of plant sulfoquinovosyldiacylglycerols by solid phase extraction and HPLC procedures.

Semi-preparative isolation of plant sulfoquinovosyldiacylglycerols by solid phase extraction and HPLC procedures.

Techniques are described for the semi-preparative isolation of sulfoquinovosyldiacylglycerol from plant leaf tissue lipid extracts and the resolution and analysis of component molecular species. Sulfoquinovosyldiacylglycerol was resolved from phospholipids in a polar lipid fraction by isocratic norm...

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Main Authors: H A Norman, C F Mischke, B Allen, J S Vincent
Format: Article
Language:English
Published: Elsevier 1996-01-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520391677
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spelling doaj-770994a0fa1842a5a9967e7c5e3585962021-04-26T05:49:35ZengElsevierJournal of Lipid Research0022-22751996-01-0137613721376Semi-preparative isolation of plant sulfoquinovosyldiacylglycerols by solid phase extraction and HPLC procedures.H A Norman0C F Mischke1B Allen2J S Vincent3Weed Science Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA.Weed Science Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA.Weed Science Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA.Weed Science Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA.Techniques are described for the semi-preparative isolation of sulfoquinovosyldiacylglycerol from plant leaf tissue lipid extracts and the resolution and analysis of component molecular species. Sulfoquinovosyldiacylglycerol was resolved from phospholipids in a polar lipid fraction by isocratic normal phase HPLC with detection at 208 nm. The mobile phase was composed of heptane-isopropanol-0.001 M KCl 40:52:8 (v/v/v). Yields from spinach leaf lipid extracts were 1.8 mg.10 g-1 fresh wt leaf tissue. Molecular species components of purified sulfoquinovosyldiacylglycerol were separated by reversed-phase C18 HPLC, and fatty acid positional distribution was defined.http://www.sciencedirect.com/science/article/pii/S0022227520391677
collection DOAJ
language English
format Article
sources DOAJ
author H A Norman
C F Mischke
B Allen
J S Vincent
spellingShingle H A Norman
C F Mischke
B Allen
J S Vincent
Semi-preparative isolation of plant sulfoquinovosyldiacylglycerols by solid phase extraction and HPLC procedures.
Journal of Lipid Research
author_facet H A Norman
C F Mischke
B Allen
J S Vincent
author_sort H A Norman
title Semi-preparative isolation of plant sulfoquinovosyldiacylglycerols by solid phase extraction and HPLC procedures.
title_short Semi-preparative isolation of plant sulfoquinovosyldiacylglycerols by solid phase extraction and HPLC procedures.
title_full Semi-preparative isolation of plant sulfoquinovosyldiacylglycerols by solid phase extraction and HPLC procedures.
title_fullStr Semi-preparative isolation of plant sulfoquinovosyldiacylglycerols by solid phase extraction and HPLC procedures.
title_full_unstemmed Semi-preparative isolation of plant sulfoquinovosyldiacylglycerols by solid phase extraction and HPLC procedures.
title_sort semi-preparative isolation of plant sulfoquinovosyldiacylglycerols by solid phase extraction and hplc procedures.
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1996-01-01
description Techniques are described for the semi-preparative isolation of sulfoquinovosyldiacylglycerol from plant leaf tissue lipid extracts and the resolution and analysis of component molecular species. Sulfoquinovosyldiacylglycerol was resolved from phospholipids in a polar lipid fraction by isocratic normal phase HPLC with detection at 208 nm. The mobile phase was composed of heptane-isopropanol-0.001 M KCl 40:52:8 (v/v/v). Yields from spinach leaf lipid extracts were 1.8 mg.10 g-1 fresh wt leaf tissue. Molecular species components of purified sulfoquinovosyldiacylglycerol were separated by reversed-phase C18 HPLC, and fatty acid positional distribution was defined.
url http://www.sciencedirect.com/science/article/pii/S0022227520391677
work_keys_str_mv AT hanorman semipreparativeisolationofplantsulfoquinovosyldiacylglycerolsbysolidphaseextractionandhplcprocedures
AT cfmischke semipreparativeisolationofplantsulfoquinovosyldiacylglycerolsbysolidphaseextractionandhplcprocedures
AT ballen semipreparativeisolationofplantsulfoquinovosyldiacylglycerolsbysolidphaseextractionandhplcprocedures
AT jsvincent semipreparativeisolationofplantsulfoquinovosyldiacylglycerolsbysolidphaseextractionandhplcprocedures
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