Semi-preparative isolation of plant sulfoquinovosyldiacylglycerols by solid phase extraction and HPLC procedures.
Techniques are described for the semi-preparative isolation of sulfoquinovosyldiacylglycerol from plant leaf tissue lipid extracts and the resolution and analysis of component molecular species. Sulfoquinovosyldiacylglycerol was resolved from phospholipids in a polar lipid fraction by isocratic norm...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1996-01-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520391677 |
| Summary: | Techniques are described for the semi-preparative isolation of sulfoquinovosyldiacylglycerol from plant leaf tissue lipid extracts and the resolution and analysis of component molecular species. Sulfoquinovosyldiacylglycerol was resolved from phospholipids in a polar lipid fraction by isocratic normal phase HPLC with detection at 208 nm. The mobile phase was composed of heptane-isopropanol-0.001 M KCl 40:52:8 (v/v/v). Yields from spinach leaf lipid extracts were 1.8 mg.10 g-1 fresh wt leaf tissue. Molecular species components of purified sulfoquinovosyldiacylglycerol were separated by reversed-phase C18 HPLC, and fatty acid positional distribution was defined. |
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| ISSN: | 0022-2275 |