The Cardiovascular and Neurotoxic Effects of the Venoms of Six Bony and Cartilaginous Fish Species
Fish venoms are often poorly studied, in part due to the difficulty in obtaining, extracting, and storing them. In this study, we characterize the cardiovascular and neurotoxic effects of the venoms from the following six species of fish: the cartilaginous stingrays Neotrygon kuhlii and Himantura to...
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doaj-76f9d9689728486f9d319aa5e30f2d312020-11-24T23:24:25ZengMDPI AGToxins2072-66512017-02-01926710.3390/toxins9020067toxins9020067The Cardiovascular and Neurotoxic Effects of the Venoms of Six Bony and Cartilaginous Fish SpeciesHan Han0Kate Baumann1Nicholas R. Casewell2Syed A. Ali3James Dobson4Ivan Koludarov5Jordan Debono6Scott C. Cutmore7Niwanthi W. Rajapakse8Timothy N. W. Jackson9Rob Jones10Wayne C. Hodgson11Bryan G. Fry12Sanjaya Kuruppu13Department of Pharmacology, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, AustraliaVenom Evolution Lab, School of Biological Sciences, University of Queensland, St Lucia, QLD 4072, AustraliaAlistair Reid Venom Research Unit, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UKDepartment of Pharmacology, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, AustraliaVenom Evolution Lab, School of Biological Sciences, University of Queensland, St Lucia, QLD 4072, AustraliaVenom Evolution Lab, School of Biological Sciences, University of Queensland, St Lucia, QLD 4072, AustraliaVenom Evolution Lab, School of Biological Sciences, University of Queensland, St Lucia, QLD 4072, AustraliaMarine Parisitology Group, School of Biological Sciences, University of Queensland, St Lucia, QLD 4072, AustraliaBaker IDI Heart and Diabetes Institute, 75 Commercial Road, Prahran 3181, AustraliaVenom Evolution Lab, School of Biological Sciences, University of Queensland, St Lucia, QLD 4072, AustraliaThe Aquarium Vet, P.O. Box 2327, Moorabbin, VIC 3189, AustraliaDepartment of Pharmacology, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, AustraliaVenom Evolution Lab, School of Biological Sciences, University of Queensland, St Lucia, QLD 4072, AustraliaDepartment of Pharmacology, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, AustraliaFish venoms are often poorly studied, in part due to the difficulty in obtaining, extracting, and storing them. In this study, we characterize the cardiovascular and neurotoxic effects of the venoms from the following six species of fish: the cartilaginous stingrays Neotrygon kuhlii and Himantura toshi, and the bony fish Platycephalus fucus, Girella tricuspidata, Mugil cephalus, and Dentex tumifrons. All venoms (10–100 μg/kg, i.v.), except G. tricuspidata and P. fuscus, induced a biphasic response on mean arterial pressure (MAP) in the anesthetised rat. P. fucus venom exhibited a hypotensive response, while venom from G. tricuspidata displayed a single depressor response. All venoms induced cardiovascular collapse at 200 μg/kg, i.v. The in vitro neurotoxic effects of venom were examined using the chick biventer cervicis nerve‐muscle (CBCNM) preparation. N. kuhlii, H. toshi, and P. fucus venoms caused concentration‐dependent inhibition of indirect twitches in the CBCNM preparation. These three venoms also inhibited responses to exogenous acetylcholine (ACh) and carbachol (CCh), but not potassium chloride (KCl), indicating a post‐synaptic mode of action. Venom from G. tricuspidata, M. cephalus, and D. tumifrons had no significant effect on indirect twitches or agonist responses in the CBCNM. Our results demonstrate that envenoming by these species of fish may result in moderate cardiovascular and/or neurotoxic effects. Future studies aimed at identifying the molecules responsible for these effects could uncover potentially novel lead compounds for future pharmaceuticals, in addition to generating new knowledge about the evolutionary relationships between venomous animals.http://www.mdpi.com/2072-6651/9/2/67venom fish cardiovascular neuromuscular toxin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Han Han Kate Baumann Nicholas R. Casewell Syed A. Ali James Dobson Ivan Koludarov Jordan Debono Scott C. Cutmore Niwanthi W. Rajapakse Timothy N. W. Jackson Rob Jones Wayne C. Hodgson Bryan G. Fry Sanjaya Kuruppu |
spellingShingle |
Han Han Kate Baumann Nicholas R. Casewell Syed A. Ali James Dobson Ivan Koludarov Jordan Debono Scott C. Cutmore Niwanthi W. Rajapakse Timothy N. W. Jackson Rob Jones Wayne C. Hodgson Bryan G. Fry Sanjaya Kuruppu The Cardiovascular and Neurotoxic Effects of the Venoms of Six Bony and Cartilaginous Fish Species Toxins venom fish cardiovascular neuromuscular toxin |
author_facet |
Han Han Kate Baumann Nicholas R. Casewell Syed A. Ali James Dobson Ivan Koludarov Jordan Debono Scott C. Cutmore Niwanthi W. Rajapakse Timothy N. W. Jackson Rob Jones Wayne C. Hodgson Bryan G. Fry Sanjaya Kuruppu |
author_sort |
Han Han |
title |
The Cardiovascular and Neurotoxic Effects of the Venoms of Six Bony and Cartilaginous Fish Species |
title_short |
The Cardiovascular and Neurotoxic Effects of the Venoms of Six Bony and Cartilaginous Fish Species |
title_full |
The Cardiovascular and Neurotoxic Effects of the Venoms of Six Bony and Cartilaginous Fish Species |
title_fullStr |
The Cardiovascular and Neurotoxic Effects of the Venoms of Six Bony and Cartilaginous Fish Species |
title_full_unstemmed |
The Cardiovascular and Neurotoxic Effects of the Venoms of Six Bony and Cartilaginous Fish Species |
title_sort |
the cardiovascular and neurotoxic effects of the venoms of six bony and cartilaginous fish species |
publisher |
MDPI AG |
series |
Toxins |
issn |
2072-6651 |
publishDate |
2017-02-01 |
description |
Fish venoms are often poorly studied, in part due to the difficulty in obtaining, extracting, and storing them. In this study, we characterize the cardiovascular and neurotoxic effects of the venoms from the following six species of fish: the cartilaginous stingrays Neotrygon kuhlii and Himantura toshi, and the bony fish Platycephalus fucus, Girella tricuspidata, Mugil cephalus, and Dentex tumifrons. All venoms (10–100 μg/kg, i.v.), except G. tricuspidata and P. fuscus, induced a biphasic response on mean arterial pressure (MAP) in the anesthetised rat. P. fucus venom exhibited a hypotensive response, while venom from G. tricuspidata displayed a single depressor response. All venoms induced cardiovascular collapse at 200 μg/kg, i.v. The in vitro neurotoxic effects of venom were examined using the chick biventer cervicis nerve‐muscle (CBCNM) preparation. N. kuhlii, H. toshi, and P. fucus venoms caused concentration‐dependent inhibition of indirect twitches in the CBCNM preparation. These three venoms also inhibited responses to exogenous acetylcholine (ACh) and carbachol (CCh), but not potassium chloride (KCl), indicating a post‐synaptic mode of action. Venom from G. tricuspidata, M. cephalus, and D. tumifrons had no significant effect on indirect twitches or agonist responses in the CBCNM. Our results demonstrate that envenoming by these species of fish may result in moderate cardiovascular and/or neurotoxic effects. Future studies aimed at identifying the molecules responsible for these effects could uncover potentially novel lead compounds for future pharmaceuticals, in addition to generating new knowledge about the evolutionary relationships between venomous animals. |
topic |
venom fish cardiovascular neuromuscular toxin |
url |
http://www.mdpi.com/2072-6651/9/2/67 |
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