Matrigel is required for efficient differentiation of isolated, stem cell-derived otic vesicles into inner ear organoids

Inner ear organoids derived from pluripotent stem cells could be a useful model system to study development, disease, and regeneration. However, there is considerable heterogeneity in the size, morphology, and efficiency of organoid production using standard protocols. Greater control of the culture...

Full description

Bibliographic Details
Main Authors:	Sarah Emily Hocevar, Liqian Liu, Robert Keith Duncan
Format:	Article
Language:	English
Published:	Elsevier 2021-05-01
Series:	Stem Cell Research
Subjects:	Inner ear Organoid Stem cell Differentiation Hair cell Matrigel
Online Access:	http://www.sciencedirect.com/science/article/pii/S1873506121001410

id	doaj-76c2d06772c14340ac12764f17d03061
record_format	Article
spelling	doaj-76c2d06772c14340ac12764f17d030612021-05-30T04:41:24ZengElsevierStem Cell Research1873-50612021-05-0153102295Matrigel is required for efficient differentiation of isolated, stem cell-derived otic vesicles into inner ear organoidsSarah Emily Hocevar0Liqian Liu1Robert Keith Duncan2Neuroscience Graduate Program, University of Michigan, Ann Arbor, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, USAKresge Hearing Research Institute, Department of Otolaryngology-Head & Neck Surgery, University of Michigan, Ann Arbor, USAKresge Hearing Research Institute, Department of Otolaryngology-Head & Neck Surgery, University of Michigan, Ann Arbor, USA; Corresponding author at: Kresge Hearing Research Institute, University of Michigan, 1150 W. Medical Center Drive, 5323 Med Sci I, Ann Arbor, MI 48109-5616, USA.Inner ear organoids derived from pluripotent stem cells could be a useful model system to study development, disease, and regeneration. However, there is considerable heterogeneity in the size, morphology, and efficiency of organoid production using standard protocols. Greater control of the culture microenvironment could decrease heterogeneity and increase the yield of organoids. Animal-derived otic vesicles show some autonomy during development and can differentiate into cochlear and vestibular domains in mesenchyme-free ex vivo culture. Therefore, we investigated whether stem cell-derived otic vesicles can autonomously generate inner ear organoids. Isolated, stem cell-derived vesicles grew into cyst-like organoids with high efficiency, over 90%, when embedded in droplets of the basement membrane matrix Matrigel. Though nearly all vesicles within the aggregate were competent to mature into organoids, the efficiency of organoid production depended on the stage of vesicle isolation and required supplementation with Matrigel.http://www.sciencedirect.com/science/article/pii/S1873506121001410Inner earOrganoidStem cellDifferentiationHair cellMatrigel
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Sarah Emily Hocevar Liqian Liu Robert Keith Duncan
spellingShingle	Sarah Emily Hocevar Liqian Liu Robert Keith Duncan Matrigel is required for efficient differentiation of isolated, stem cell-derived otic vesicles into inner ear organoids Stem Cell Research Inner ear Organoid Stem cell Differentiation Hair cell Matrigel
author_facet	Sarah Emily Hocevar Liqian Liu Robert Keith Duncan
author_sort	Sarah Emily Hocevar
title	Matrigel is required for efficient differentiation of isolated, stem cell-derived otic vesicles into inner ear organoids
title_short	Matrigel is required for efficient differentiation of isolated, stem cell-derived otic vesicles into inner ear organoids
title_full	Matrigel is required for efficient differentiation of isolated, stem cell-derived otic vesicles into inner ear organoids
title_fullStr	Matrigel is required for efficient differentiation of isolated, stem cell-derived otic vesicles into inner ear organoids
title_full_unstemmed	Matrigel is required for efficient differentiation of isolated, stem cell-derived otic vesicles into inner ear organoids
title_sort	matrigel is required for efficient differentiation of isolated, stem cell-derived otic vesicles into inner ear organoids
publisher	Elsevier
series	Stem Cell Research
issn	1873-5061
publishDate	2021-05-01
description	Inner ear organoids derived from pluripotent stem cells could be a useful model system to study development, disease, and regeneration. However, there is considerable heterogeneity in the size, morphology, and efficiency of organoid production using standard protocols. Greater control of the culture microenvironment could decrease heterogeneity and increase the yield of organoids. Animal-derived otic vesicles show some autonomy during development and can differentiate into cochlear and vestibular domains in mesenchyme-free ex vivo culture. Therefore, we investigated whether stem cell-derived otic vesicles can autonomously generate inner ear organoids. Isolated, stem cell-derived vesicles grew into cyst-like organoids with high efficiency, over 90%, when embedded in droplets of the basement membrane matrix Matrigel. Though nearly all vesicles within the aggregate were competent to mature into organoids, the efficiency of organoid production depended on the stage of vesicle isolation and required supplementation with Matrigel.
topic	Inner ear Organoid Stem cell Differentiation Hair cell Matrigel
url	http://www.sciencedirect.com/science/article/pii/S1873506121001410
work_keys_str_mv	AT sarahemilyhocevar matrigelisrequiredforefficientdifferentiationofisolatedstemcellderivedoticvesiclesintoinnerearorganoids AT liqianliu matrigelisrequiredforefficientdifferentiationofisolatedstemcellderivedoticvesiclesintoinnerearorganoids AT robertkeithduncan matrigelisrequiredforefficientdifferentiationofisolatedstemcellderivedoticvesiclesintoinnerearorganoids
_version_	1721421089074053120

Matrigel is required for efficient differentiation of isolated, stem cell-derived otic vesicles into inner ear organoids

Similar Items