Mycoplasma detection by triplex real-time PCR in bronchoalveolar lavage fluid from bovine respiratory disease complex cases

Abstract Background In this study we evaluated the RespoCheck Mycoplasma triplex real-time PCR for the detection in bronchoalveolar lavage fluid (BALF) of Mycoplasma (M.) dispar, M. bovis and M. bovirhinis, all three associated with bovine respiratory disease (BRD). Primers and probes of the RespoCh...

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Main Authors: Jan B. W. J. Cornelissen, Freddy M. de Bree, Fimme J. van der Wal, Engbert A. Kooi, Miriam G. J. Koene, Alex Bossers, Bregtje Smid, Adriaan F. Antonis, Henk J. Wisselink
Format: Article
Language:English
Published: BMC 2017-04-01
Series:BMC Veterinary Research
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12917-017-1023-6