Double-setting alpha-tricalcium phosphate cement provided with interconection channels in rabbits after enucleation: A potential implant for the anophthalmic socket

• Main Authors: D.C. Gomes Junior, A.P. Oriá, F.A. Dórea Neto, L.A. dos Santos, A. Estrela-Lima, V.F. Barbosa, J.M. Costa Neto, J.L. Laus, C.R. Daleck
• Format: Article
• Language: English
• Published: Universidade Federal de Minas Gerais 2015-02-01
• Series: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
• Subjects: implante; olho; prótese
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000100119&lng=en&tlng=en

The purpose of this study was to evaluate the macroscopy and microstructure of a double setting alpha-tricalcium phosphate bone cement sphere provided with interconnection channels (alpha-TCP-i), as well as the integration of the implant with the rabbits' orbital tissue, through macroscopic analysis and histopathology. The external and internal surfaces of the alpha-TCP-i were evaluated macroscopically and by electron microscopy. Twelve New Zealand rabbits received 12mm implants of alpha-TCP-i following enucleation of the left eye. The clinical assessment was undertaken daily during the first 15 days, followed by fortnightly assessment until the end of the study period. For the morphological analysis, exenteration was performed in 3 animals per experimental period (15, 45, 90 and 180 days). The external and internal surfaces of the implant appeared solid, smooth and compact, with six channels which interconnected centrally. The micro-architecture was characterized by the formation of columns of hexagonal crystals. No signs of infection, exposure, dehiscence of sutures or extrusion of the implant were noted in any of the animals during the entire period of the study. The morphological evaluation demonstrated the presence of a thin capsule around the implant, from whence appeared fibro-vascular projections, which penetrated it through the interconnecting channels. In the first days after the insertion of the implant, an intense inflammatory reaction was noted. At 180 days, however, there were no signs of inflammation. The alpha-tricalcium phosphate cement implant was well tolerated in this rabbit model and appeared to be relatively inert with some fibrovascular ingrowth through the large channels.