Nanoblinker: Brownian motion powered bio-nanomachine for FRET detection of phagocytic phase of apoptosis.
We describe a new type of bio-nanomachine which runs on thermal noise. The machine is solely powered by the random motion of water molecules in its environment and does not ever require re-fuelling. The construct, which is made of DNA and vaccinia virus topoisomerase protein, can detect DNA damage b...
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doaj-765ec10a96584f21a04e70696dd71aff2020-11-24T22:04:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0199e10873410.1371/journal.pone.0108734Nanoblinker: Brownian motion powered bio-nanomachine for FRET detection of phagocytic phase of apoptosis.Candace L MinchewVladimir V DidenkoWe describe a new type of bio-nanomachine which runs on thermal noise. The machine is solely powered by the random motion of water molecules in its environment and does not ever require re-fuelling. The construct, which is made of DNA and vaccinia virus topoisomerase protein, can detect DNA damage by employing fluorescence. It uses Brownian motion as a cyclic motor to continually separate and bring together two types of fluorescent hairpins participating in FRET. This bio-molecular oscillator is a fast and specific sensor of 5'OH double-strand DNA breaks present in phagocytic phase of apoptosis. The detection takes 30 s in solution and 3 min in cell suspensions. The phagocytic phase is critical for the effective execution of apoptosis as it ensures complete degradation of the dying cells' DNA, preventing release of pathological, viral and tumor DNA and self-immunization. The construct can be used as a smart FRET probe in studies of cell death and phagocytosis.http://europepmc.org/articles/PMC4182547?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Candace L Minchew Vladimir V Didenko |
spellingShingle |
Candace L Minchew Vladimir V Didenko Nanoblinker: Brownian motion powered bio-nanomachine for FRET detection of phagocytic phase of apoptosis. PLoS ONE |
author_facet |
Candace L Minchew Vladimir V Didenko |
author_sort |
Candace L Minchew |
title |
Nanoblinker: Brownian motion powered bio-nanomachine for FRET detection of phagocytic phase of apoptosis. |
title_short |
Nanoblinker: Brownian motion powered bio-nanomachine for FRET detection of phagocytic phase of apoptosis. |
title_full |
Nanoblinker: Brownian motion powered bio-nanomachine for FRET detection of phagocytic phase of apoptosis. |
title_fullStr |
Nanoblinker: Brownian motion powered bio-nanomachine for FRET detection of phagocytic phase of apoptosis. |
title_full_unstemmed |
Nanoblinker: Brownian motion powered bio-nanomachine for FRET detection of phagocytic phase of apoptosis. |
title_sort |
nanoblinker: brownian motion powered bio-nanomachine for fret detection of phagocytic phase of apoptosis. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
We describe a new type of bio-nanomachine which runs on thermal noise. The machine is solely powered by the random motion of water molecules in its environment and does not ever require re-fuelling. The construct, which is made of DNA and vaccinia virus topoisomerase protein, can detect DNA damage by employing fluorescence. It uses Brownian motion as a cyclic motor to continually separate and bring together two types of fluorescent hairpins participating in FRET. This bio-molecular oscillator is a fast and specific sensor of 5'OH double-strand DNA breaks present in phagocytic phase of apoptosis. The detection takes 30 s in solution and 3 min in cell suspensions. The phagocytic phase is critical for the effective execution of apoptosis as it ensures complete degradation of the dying cells' DNA, preventing release of pathological, viral and tumor DNA and self-immunization. The construct can be used as a smart FRET probe in studies of cell death and phagocytosis. |
url |
http://europepmc.org/articles/PMC4182547?pdf=render |
work_keys_str_mv |
AT candacelminchew nanoblinkerbrownianmotionpoweredbionanomachineforfretdetectionofphagocyticphaseofapoptosis AT vladimirvdidenko nanoblinkerbrownianmotionpoweredbionanomachineforfretdetectionofphagocyticphaseofapoptosis |
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