Wide-field polygon-scanning photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate

We report wide-field polygon-scanning functional OR-PAM that for the first time achieves 1-MHz A-line rate of oxygen saturation in vivo. We address two technical challenges. The first is a 1-MHz dual-wavelength pulsed laser that has sufficient pulse energy and ultrafast wavelength switching. The sec...

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Main Authors:	Jiangbo Chen, Yachao Zhang, Linyun He, Yizhi Liang, Lidai Wang
Format:	Article
Language:	English
Published:	Elsevier 2020-12-01
Series:	Photoacoustics
Subjects:	Functional imaging Photoacoustic microscopy Wide-Field Oxygen saturation
Online Access:	http://www.sciencedirect.com/science/article/pii/S2213597920300355

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record_format	Article
spelling	doaj-764fede682de4a589b796070dadd90b82021-01-02T05:09:40ZengElsevierPhotoacoustics2213-59792020-12-0120100195Wide-field polygon-scanning photoacoustic microscopy of oxygen saturation at 1-MHz A-line rateJiangbo Chen0Yachao Zhang1Linyun He2Yizhi Liang3Lidai Wang4Department of Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Ave, Kowloon, Hong Kong SAR, ChinaDepartment of Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Ave, Kowloon, Hong Kong SAR, ChinaDepartment of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, ChinaGuangdong Provincial Key Laboratory of Optical Fiber Sensing and Communications, Institute of Photonics Technology, Jinan University, Guangzhou, 510632, China; Corresponding author.Department of Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Ave, Kowloon, Hong Kong SAR, China; City University of Hong Kong Shenzhen Research Institute, Yuexing Yi Dao, Shenzhen, Guang Dong, 518057, China; Corresponding author at: Department of Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Ave, Kowloon, Hong Kong SAR, China.We report wide-field polygon-scanning functional OR-PAM that for the first time achieves 1-MHz A-line rate of oxygen saturation in vivo. We address two technical challenges. The first is a 1-MHz dual-wavelength pulsed laser that has sufficient pulse energy and ultrafast wavelength switching. The second is a polygon-scanning imaging probe that has a fast scanning speed, a large field of view, and great sensitivity. The OR-PAM system offers a B-scan rate of 477.5 Hz in a 12-mm range and a volumetric imaging rate of ∼1 Hz over a 12 × 5 mm2 scanning area. We image microvasculature and blood oxygen saturation in a 12 × 12 mm2 scanning area in 5 s. Dynamic imaging of oxygen saturation in the mouse ear is demonstrated to monitor fast response to epinephrine injection. The new wide-field fast functional imaging ability broadens the biomedical application of OR-PAM.http://www.sciencedirect.com/science/article/pii/S2213597920300355Functional imagingPhotoacoustic microscopyWide-FieldOxygen saturation
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Jiangbo Chen Yachao Zhang Linyun He Yizhi Liang Lidai Wang
spellingShingle	Jiangbo Chen Yachao Zhang Linyun He Yizhi Liang Lidai Wang Wide-field polygon-scanning photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate Photoacoustics Functional imaging Photoacoustic microscopy Wide-Field Oxygen saturation
author_facet	Jiangbo Chen Yachao Zhang Linyun He Yizhi Liang Lidai Wang
author_sort	Jiangbo Chen
title	Wide-field polygon-scanning photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate
title_short	Wide-field polygon-scanning photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate
title_full	Wide-field polygon-scanning photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate
title_fullStr	Wide-field polygon-scanning photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate
title_full_unstemmed	Wide-field polygon-scanning photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate
title_sort	wide-field polygon-scanning photoacoustic microscopy of oxygen saturation at 1-mhz a-line rate
publisher	Elsevier
series	Photoacoustics
issn	2213-5979
publishDate	2020-12-01
description	We report wide-field polygon-scanning functional OR-PAM that for the first time achieves 1-MHz A-line rate of oxygen saturation in vivo. We address two technical challenges. The first is a 1-MHz dual-wavelength pulsed laser that has sufficient pulse energy and ultrafast wavelength switching. The second is a polygon-scanning imaging probe that has a fast scanning speed, a large field of view, and great sensitivity. The OR-PAM system offers a B-scan rate of 477.5 Hz in a 12-mm range and a volumetric imaging rate of ∼1 Hz over a 12 × 5 mm2 scanning area. We image microvasculature and blood oxygen saturation in a 12 × 12 mm2 scanning area in 5 s. Dynamic imaging of oxygen saturation in the mouse ear is demonstrated to monitor fast response to epinephrine injection. The new wide-field fast functional imaging ability broadens the biomedical application of OR-PAM.
topic	Functional imaging Photoacoustic microscopy Wide-Field Oxygen saturation
url	http://www.sciencedirect.com/science/article/pii/S2213597920300355
work_keys_str_mv	AT jiangbochen widefieldpolygonscanningphotoacousticmicroscopyofoxygensaturationat1mhzalinerate AT yachaozhang widefieldpolygonscanningphotoacousticmicroscopyofoxygensaturationat1mhzalinerate AT linyunhe widefieldpolygonscanningphotoacousticmicroscopyofoxygensaturationat1mhzalinerate AT yizhiliang widefieldpolygonscanningphotoacousticmicroscopyofoxygensaturationat1mhzalinerate AT lidaiwang widefieldpolygonscanningphotoacousticmicroscopyofoxygensaturationat1mhzalinerate
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Wide-field polygon-scanning photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate

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