Validation of a Reversed-Phase High Performance Liquid Chromatography Method for the Simultaneous Analysis of Cysteine and Reduced Glutathione in Mouse Organs

Validation of a Reversed-Phase High Performance Liquid Chromatography Method for the Simultaneous Analysis of Cysteine and Reduced Glutathione in Mouse Organs

A depletion of reduced glutathione (GSH) has been observed in pathological conditions and in aging. Measuring GSH in tissues using mouse models is an excellent way to assess GSH depletion and the potential therapeutic efficacy of drugs used to maintain and/or restore cellular redox potential. A high...

Full description

Bibliographic Details
Main Authors:	Serena Brundu, Lucia Nencioni, Ignacio Celestino, Paolo Coluccio, Anna Teresa Palamara, Mauro Magnani, Alessandra Fraternale
Format:	Article
Language:	English
Published:	Hindawi Limited 2016-01-01
Series:	Oxidative Medicine and Cellular Longevity
Online Access:	http://dx.doi.org/10.1155/2016/1746985

Similar Items

Activation of NRF2 and ATF4 Signaling by the Pro-Glutathione Molecule I-152, A Co-Drug of <i>N</i>-Acetyl-Cysteine and Cysteamine
by: Rita Crinelli, et al.
Published: (2021-01-01)

Redox-Modulating Agents in the Treatment of Viral Infections
by: Paola Checconi, et al.
Published: (2020-06-01)

Boosting GSH Using the Co-Drug Approach: I-152, a Conjugate of <i>N</i>-acetyl-cysteine and β-mercaptoethylamine
by: Rita Crinelli, et al.
Published: (2019-06-01)

Cysteine, Glutathione, and Thiol Redox Balance in Astrocytes
by: Gethin J. McBean
Published: (2017-08-01)

Kinetics and Mechanism of Reduction of Coordinated Superoxide with Glutathione and Cysteine
by: Hsin-Yi Wang, et al.
Published: (2016)

Intracellular Redox-Modulated Pathways as Targets for Effective Approaches in the Treatment of Viral Infection
by: Alessandra Fraternale, et al.
Published: (2021-03-01)

The still mysterious roles of cysteine-containing glutathione transferases in plants
by: Pierre-Alexandre eLallement, et al.
Published: (2014-08-01)

Toxicity and safety study of silver and gold nanoparticles functionalized with cysteine and glutathione
by: Barbara Pem, et al.
Published: (2019-09-01)

Cysteine, Malondyaldehide (MDA) and Glutathione (GSH) Levels in Marasmic Type Malnutrition
by: Dwi Indah Cahyani, et al.
Published: (2017-06-01)

Glutathione provides a source of cysteine essential for intracellular multiplication of Francisella tularensis.
by: Khaled Alkhuder, et al.
Published: (2009-01-01)

Cord blood glutathione depletion in preterm infants: correlation with maternal cysteine depletion.
by: Alice Küster, et al.
Published: (2011-01-01)

Comparison of Glutathione, Cysteine, and their Redox Potentials in the Plasma of Critically Ill and Healthy Children
by: Jocelyn Rebecca Grunwell, et al.
Published: (2015-05-01)

Electrochemical Determination of Cysteine and Glutathione on the Nanocomposite Modified Screen–Printed Carbon Electrodes
by: Ya-Ping Hsiao, et al.
Published: (2010)

Glutathione Fine-Tunes the Innate Immune Response toward Antiviral Pathways in a Macrophage Cell Line Independently of Its Antioxidant Properties
by: Marina Diotallevi, et al.
Published: (2017-09-01)

Glutathione and glutathione S-transferases in chlorambucil resistant mouse cell lines
by: Yang, Wen Z.
Published: (2013)

Glutathione and glutathione S-transferases in chlorambucil resistant mouse cell lines
by: Yang, Wen Z.
Published: (2013)

Spermatogonial Gene Networks Selectively Couple to Glutathione and Pentose Phosphate Metabolism but Not Cysteine Biosynthesis
by: David Prokai, et al.
Published: (2021-01-01)

Metabolism and Bioactivation of Corynoline With Characterization of the Glutathione/Cysteine Conjugate and Evaluation of Its Hepatotoxicity in Mice
by: Ruijuan Liu, et al.
Published: (2018-11-01)

Neuregulin 1 Promotes Glutathione-Dependent Neuronal Cobalamin Metabolism by Stimulating Cysteine Uptake
by: Yiting Zhang, et al.
Published: (2016-01-01)

A Fluorescent Cy7-Mercaptopyridine for the Selective Detection of Glutathione over Homocysteine and Cysteine
by: Shin A Yoon, et al.
Published: (2018-09-01)

The Antioxidant Role of Glutathione and N-Acetyl-Cysteine Supplements and Exercise-Induced Oxidative Stress
by: Willoughby Darryn, et al.
Published: (2005-12-01)

Structural/functional study of cysteine residues on rat liver glutathione S-transferase 3-3
by: XIE,ZHI-CEHNG, et al.
Published: (1991)

Enzymatic "Click" Ligation: Selective Cysteine Modification in Polypeptides Enabled by Promiscuous Glutathione S-Transferase
by: Zhang, Chi, et al.
Published: (2015)

The Effects of Antioxidants, N-acetyl-L-cystein, N-acetyl-L-cystein Amide, Glutathione or Cysteamine on the Development of in vitro Fertilized bovine Oocytes
by: Min-Su Kim, et al.
Published: (2017-09-01)

Effect of the active principle- Sallyl cysteine and its analogue S-methyl cysteine -in aged garlic on the glutathione-related metabolism system of primary rat hepatocytes
by: Yang, Chou-Chin, et al.
Published: (1997)

Red Blood Cell Membrane Processing for Biomedical Applications
by: Luigia Rossi, et al.
Published: (2019-08-01)

Simultaneous determination of arginine, citrulline, and asymmetric dimethylarginine in plasma by reverse-phase high-performance liquid chromatography
by: P. V. L. N Srinivasa Rao, et al.
Published: (2017-10-01)

Effective refolding of a cysteine rich glycoside hydrolase family 19 recombinant chitinase from Streptomyces griseus by reverse dilution and affinity chromatography.
by: Ayokunmi Omolola Oyeleye, et al.
Published: (2020-01-01)

Regulation of mouse hepatic glutathione S-transferases
by: Dolan, Catherine
Published: (1991)

GSH-C4 Acts as Anti-inflammatory Drug in Different Models of Canonical and Cell Autonomous Inflammation Through NFκB Inhibition
by: Dolores Limongi, et al.
Published: (2019-02-01)

Corrigendum: GSH-C4 Acts as Anti-inflammatory Drug in Different Models of Canonical and Cell Autonomous Inflammation Through NFκB Inhibition
by: Dolores Limongi, et al.
Published: (2019-07-01)

The study of cysteine levels in hair products using high-performance liquid chromatography
by: Lyu-An Chen, et al.
Published: (2014)

Simultaneous determination of ascorbic acid and caffeine in commercial soft drinks using reversed-phase ultraperformance liquid chromatography
by: Fatma Turak, et al.
Published: (2017-04-01)

In vitro effect of N-acetyl-L-cysteine on glutathione and sulfhydryl levels in X-linked adrenoleukodystrophy patients
by: Desirèe Padilha Marchetti, et al.
Published: (2017-04-01)

In vitro effect of N-acetyl-L-cysteine on glutathione and sulfhydryl levels in X-linked adrenoleukodystrophy patients
by: Desirèe Padilha Marchetti, et al.
Published: (2017-04-01)

Molecules altering the intracellular thiol content modulate NF-kB and STAT-1/IRF-1 signalling pathways and IL-12 p40 and IL-27 p28 production in murine macrophages.
by: Alessandra Fraternale, et al.
Published: (2013-01-01)

Advances on plant redox proteomics technologies for cysteine reversible oxidation
by: WANG Lin, et al.
Published: (2018-10-01)

Human glutathione-S-transferase pi potentiates the cysteine-protease activity of the Der p 1 allergen from house dust mite through a cysteine redox mechanism
by: Juan Carlos López-Rodríguez, et al.
Published: (2019-09-01)

Involvement of <i>L</i>-Cysteine Desulfhydrase and Hydrogen Sulfide in Glutathione-Induced Tolerance to Salinity by Accelerating Ascorbate-Glutathione Cycle and Glyoxalase System in <i>Capsicum</i>
by: Cengiz Kaya, et al.
Published: (2020-07-01)

Analytical method development and validation of simultaneous estimation of rabeprazole, pantoprazole, and itopride by reverse-phase high-performance liquid chromatography
by: Senthamil Selvan Perumal, et al.
Published: (2014-12-01)