Proteomic analysis of energy metabolism and signal transduction in irradiated melanoma cells

Proteomic analysis of energy metabolism and signal transduction in irradiated melanoma cells

AIM: To analyze proteomic and signal transduction alterations in irradiated melanoma cells. METHODS: We combined stable isotope labeling with amino acids in cell culture (SILAC) with highly sensitive shotgun tandem mass spectrometry (MS) to create an efficient approach for protein quantification. Pr...

Full description

Bibliographic Details
Main Authors: Yang Shen, Yi-Lan Sun, Zhi-Tong Bing, Kai Shi, Lu-Bin Yan
Format: Article
Language:English
Published: Press of International Journal of Ophthalmology (IJO PRESS) 2013-06-01
Series:International Journal of Ophthalmology
Subjects:
melanoma cell
2D-LC-MS/MS
stable isotope labeling with amino acids
proteomic analysis
X-ray irradiation
protein-protein interaction
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3693007/

Internet

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3693007/

Similar Items