Comparison of Molecular (PCR) and Pap Smear Methods in Diagnosis of Human Papiloma Virus (HPV) in Women with Genital Warts

Abstract Background and Objective: Much research has shown that Human Papiloma Virus (HPV) plays an important role in cervix cancer and it is the cause of 99% of cervix cancer worldwide. Lots of research has been done to find a proper method for HPV diagnosis and screening in patients with genital...

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Main Authors:	H Bashi zadeh Fakhar, R Faraji, M Ghane, M Jafarpour, B Ashoorizadeh
Format:	Article
Language:	English
Published:	Golestan University of Medical Sciences 2013-10-01
Series:	Medical Laboratory Journal
Online Access:	http://mlj.goums.ac.ir/article-1-300-en.html

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spelling	doaj-760c7793f46245bdbfa1dd7059cecfa22021-09-01T13:20:25ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492013-10-0173915Comparison of Molecular (PCR) and Pap Smear Methods in Diagnosis of Human Papiloma Virus (HPV) in Women with Genital WartsH Bashi zadeh Fakhar0R Faraji1M Ghane2M Jafarpour3B Ashoorizadeh4 MSc of Microbiology, Young Researchers Club, Islamic Azad University Associated Professor of Gynecology, Department of Gynecology Assistant Professor of Microbiology, Department of Microbiology, Islamic Azad University Assistant Professor of Microbiology, Department of Microbiology, Islamic Azad University Resident of Surgery, Department of Surgery Abstract Background and Objective: Much research has shown that Human Papiloma Virus (HPV) plays an important role in cervix cancer and it is the cause of 99% of cervix cancer worldwide. Lots of research has been done to find a proper method for HPV diagnosis and screening in patients with genital warts. This study aimed at comparing PCR method with Pap smear test in HPV screening. Material and Methods: Considering the presence of DNA of HPV, 45 vaginal and cervix swap samples of women with genital warts were tested by means of specific PCR and Pap smear from September 2010 to April 2011. Results: Out of 45 vaginal and cervix swap samples of women suffering genital warts, 37 samples (82.2%) are positive. Of 45 Pap smear samples, 13 (29%) are neoplasia and 32 (71%) normal. Conclusion: The difference between the results of PCR and Pap smear is due to low specification and sensitivity of Pap smear. Thus it is recommended using diagnostic PCR method in addition to Pap smear in order to promote the quality of screening in individuals with genital warts. Keywords: Human Papiloma Virus (HPV) Genital Warts Molecular (PCR) Pap Smearhttp://mlj.goums.ac.ir/article-1-300-en.html
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	H Bashi zadeh Fakhar R Faraji M Ghane M Jafarpour B Ashoorizadeh
spellingShingle	H Bashi zadeh Fakhar R Faraji M Ghane M Jafarpour B Ashoorizadeh Comparison of Molecular (PCR) and Pap Smear Methods in Diagnosis of Human Papiloma Virus (HPV) in Women with Genital Warts Medical Laboratory Journal
author_facet	H Bashi zadeh Fakhar R Faraji M Ghane M Jafarpour B Ashoorizadeh
author_sort	H Bashi zadeh Fakhar
title	Comparison of Molecular (PCR) and Pap Smear Methods in Diagnosis of Human Papiloma Virus (HPV) in Women with Genital Warts
title_short	Comparison of Molecular (PCR) and Pap Smear Methods in Diagnosis of Human Papiloma Virus (HPV) in Women with Genital Warts
title_full	Comparison of Molecular (PCR) and Pap Smear Methods in Diagnosis of Human Papiloma Virus (HPV) in Women with Genital Warts
title_fullStr	Comparison of Molecular (PCR) and Pap Smear Methods in Diagnosis of Human Papiloma Virus (HPV) in Women with Genital Warts
title_full_unstemmed	Comparison of Molecular (PCR) and Pap Smear Methods in Diagnosis of Human Papiloma Virus (HPV) in Women with Genital Warts
title_sort	comparison of molecular (pcr) and pap smear methods in diagnosis of human papiloma virus (hpv) in women with genital warts
publisher	Golestan University of Medical Sciences
series	Medical Laboratory Journal
issn	2538-4449
publishDate	2013-10-01
description	Abstract Background and Objective: Much research has shown that Human Papiloma Virus (HPV) plays an important role in cervix cancer and it is the cause of 99% of cervix cancer worldwide. Lots of research has been done to find a proper method for HPV diagnosis and screening in patients with genital warts. This study aimed at comparing PCR method with Pap smear test in HPV screening. Material and Methods: Considering the presence of DNA of HPV, 45 vaginal and cervix swap samples of women with genital warts were tested by means of specific PCR and Pap smear from September 2010 to April 2011. Results: Out of 45 vaginal and cervix swap samples of women suffering genital warts, 37 samples (82.2%) are positive. Of 45 Pap smear samples, 13 (29%) are neoplasia and 32 (71%) normal. Conclusion: The difference between the results of PCR and Pap smear is due to low specification and sensitivity of Pap smear. Thus it is recommended using diagnostic PCR method in addition to Pap smear in order to promote the quality of screening in individuals with genital warts. Keywords: Human Papiloma Virus (HPV) Genital Warts Molecular (PCR) Pap Smear
url	http://mlj.goums.ac.ir/article-1-300-en.html
work_keys_str_mv	AT hbashizadehfakhar comparisonofmolecularpcrandpapsmearmethodsindiagnosisofhumanpapilomavirushpvinwomenwithgenitalwarts AT rfaraji comparisonofmolecularpcrandpapsmearmethodsindiagnosisofhumanpapilomavirushpvinwomenwithgenitalwarts AT mghane comparisonofmolecularpcrandpapsmearmethodsindiagnosisofhumanpapilomavirushpvinwomenwithgenitalwarts AT mjafarpour comparisonofmolecularpcrandpapsmearmethodsindiagnosisofhumanpapilomavirushpvinwomenwithgenitalwarts AT bashoorizadeh comparisonofmolecularpcrandpapsmearmethodsindiagnosisofhumanpapilomavirushpvinwomenwithgenitalwarts
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Comparison of Molecular (PCR) and Pap Smear Methods in Diagnosis of Human Papiloma Virus (HPV) in Women with Genital Warts

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