Pomegranate juice (punica granatum): a new storage medium for avulsed teeth.

• Main Authors: Sara Tavassoli-Hojjati, Elham Aliasghar, Fatemeh Ahmadian Babaki, Fatemeh Emadi, Maliheh Parsa, Shohreh Tavajohi, Maryam Ahmadyar, Seyed Nasser Ostad
• Format: Article
• Language: English
• Published: Tehran University of Medical Sciences 2014-04-01
• Series: Frontiers in Dentistry
• Subjects: Cell Culture; Neutral Red; Periodontal Ligament; Punica Granatum; Tooth Avulsion
• Online Access: https://jdt.tums.ac.ir/index.php/jdt/article/view/513

There is evidence indicating that pomegranate juice contains many of the essential properties necessary to retain cell viability and cell proliferation. These properties indicate that pomegranate juice is a suitable storage medium for avulsed teeth. However, this idea has not yet been tested. In this study, the capacity of pomegranate juice (PJ) as a storage medium for retaining avulsed teeth was evaluated.PDL fibroblasts were obtained from healthy human premolars and cultured in Dulbecco's Modified Eagle's Medium (DMEM). Cultured cells were subjected to different concentrations of pomegranate juice (PJ), 1% Hank's balanced salt solution (HBSS) and tap water for 1, 3, 6 and 24 hours. PDL cell viability was assessed by the neutral red uptake assay.The results indicated that 7.5% PJ was the most effective solution for maintaining PDL cell viability amongst all the experimental solution's and time intervals (P