Hemolysin gene detection in some isolates of Klebsiella pneumonia by PCR

Hemolysin gene detection in some isolates of Klebsiella pneumonia by PCR

Hemolysin protein is exotoxin produced by organisms that cause lysis of blood cells. This study was conducted to screen the presence of hemolysin gene from 20 isolates of Klebsiella pneumonia based 16S rRNA genes by using a specific primer. This gene potent the pathogenesis of Klebsiella pneumonia....

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Main Authors: Jameela Radi Esmaeel, Jenan Nadhim Sadeq
Format: Article
Language:English
Published: College of Veterinary Medicine 2018-12-01
Series:Al-Qadisiyah Journal of Veterinary Medicine Sciences
Online Access:http://www.qu.edu.iq/journalvm/index.php/vm_journal/article/view/504
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spelling doaj-758d3c04b31744a780ad99d2d909074e2020-11-24T22:43:29ZengCollege of Veterinary MedicineAl-Qadisiyah Journal of Veterinary Medicine Sciences 1818-57462313-44292018-12-01172495210.29079/vol17iss2art504Hemolysin gene detection in some isolates of Klebsiella pneumonia by PCRJameela Radi Esmaeel0Jenan Nadhim Sadeq1College of Veterinary Medicine, University of Al-Qadisiyah, IraqCollege of Veterinary Medicine, University of Al-Qadisiyah, IraqHemolysin protein is exotoxin produced by organisms that cause lysis of blood cells. This study was conducted to screen the presence of hemolysin gene from 20 isolates of Klebsiella pneumonia based 16S rRNA genes by using a specific primer. This gene potent the pathogenesis of Klebsiella pneumonia. The primer was designed in this study by NCBI-GenBank and primer3 plus. (Bioneer Company provided the primers. Korea). Molecular detection of isolates, which give away specific PCR products of 505bp for hly gene, hemolysin gene, was detected in 70% (14/20). http://www.qu.edu.iq/journalvm/index.php/vm_journal/article/view/504
collection DOAJ
language English
format Article
sources DOAJ
author Jameela Radi Esmaeel
Jenan Nadhim Sadeq
spellingShingle Jameela Radi Esmaeel
Jenan Nadhim Sadeq
Hemolysin gene detection in some isolates of Klebsiella pneumonia by PCR
Al-Qadisiyah Journal of Veterinary Medicine Sciences
author_facet Jameela Radi Esmaeel
Jenan Nadhim Sadeq
author_sort Jameela Radi Esmaeel
title Hemolysin gene detection in some isolates of Klebsiella pneumonia by PCR
title_short Hemolysin gene detection in some isolates of Klebsiella pneumonia by PCR
title_full Hemolysin gene detection in some isolates of Klebsiella pneumonia by PCR
title_fullStr Hemolysin gene detection in some isolates of Klebsiella pneumonia by PCR
title_full_unstemmed Hemolysin gene detection in some isolates of Klebsiella pneumonia by PCR
title_sort hemolysin gene detection in some isolates of klebsiella pneumonia by pcr
publisher College of Veterinary Medicine
series Al-Qadisiyah Journal of Veterinary Medicine Sciences
issn 1818-5746
2313-4429
publishDate 2018-12-01
description Hemolysin protein is exotoxin produced by organisms that cause lysis of blood cells. This study was conducted to screen the presence of hemolysin gene from 20 isolates of Klebsiella pneumonia based 16S rRNA genes by using a specific primer. This gene potent the pathogenesis of Klebsiella pneumonia. The primer was designed in this study by NCBI-GenBank and primer3 plus. (Bioneer Company provided the primers. Korea). Molecular detection of isolates, which give away specific PCR products of 505bp for hly gene, hemolysin gene, was detected in 70% (14/20).
url http://www.qu.edu.iq/journalvm/index.php/vm_journal/article/view/504
work_keys_str_mv AT jameelaradiesmaeel hemolysingenedetectioninsomeisolatesofklebsiellapneumoniabypcr
AT jenannadhimsadeq hemolysingenedetectioninsomeisolatesofklebsiellapneumoniabypcr
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