The Influence of Electron Beam Sterilization on In Vivo Degradation of β-TCP/PCL of Different Composite Ratios for Bone Tissue Engineering
We evaluated the effect of electron beam (E-beam) sterilization (25 kGy, ISO 11137) on the degradation of β-tricalcium phosphate/polycaprolactone (β-TCP/PCL) composite filaments of various ratios (0:100, 20:80, 40:60, and 60:40 TCP:PCL by mass) in a rat subcutaneous model for 24 we...
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doaj-7570a30b672549e6b4cb19c1b653b4962020-11-25T02:15:07ZengMDPI AGMicromachines2072-666X2020-03-0111327310.3390/mi11030273mi11030273The Influence of Electron Beam Sterilization on In Vivo Degradation of β-TCP/PCL of Different Composite Ratios for Bone Tissue EngineeringJin-Ho Kang0Janelle Kaneda1Jae-Gon Jang2Kumaresan Sakthiabirami3Elaine Lui4Carolyn Kim5Aijun Wang6Sang-Won Park7Yunzhi Peter Yang8Department of Prosthodontics, School of Dentistry, Chonnam National University, Gwanju 61186, KoreaDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USADepartment of Prosthodontics, School of Dentistry, Chonnam National University, Gwanju 61186, KoreaDepartment of Prosthodontics, School of Dentistry, Chonnam National University, Gwanju 61186, KoreaDepartment of Mechanical Engineering, Stanford University, Stanford, CA 94305, USADepartment of Mechanical Engineering, Stanford University, Stanford, CA 94305, USASurgical Bioengineering Laboratory, Department of Surgery, School of Medicine, University of California–Davis, Sacramento, CA 95817, USADepartment of Prosthodontics, School of Dentistry, Chonnam National University, Gwanju 61186, KoreaDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USAWe evaluated the effect of electron beam (E-beam) sterilization (25 kGy, ISO 11137) on the degradation of β-tricalcium phosphate/polycaprolactone (β-TCP/PCL) composite filaments of various ratios (0:100, 20:80, 40:60, and 60:40 TCP:PCL by mass) in a rat subcutaneous model for 24 weeks. Volumes of the samples before implantation and after explantation were measured using micro-computed tomography (micro-CT). The filament volume changes before sacrifice were also measured using a live micro-CT. In our micro-CT analyses, there was no significant difference in volume change between the E-beam treated groups and non-E-beam treated groups of the same β-TCP to PCL ratios, except for the 0% β-TCP group. However, the average volume reduction differences between the E-beam and non-E-beam groups in the same-ratio samples were 0.76% (0% TCP), 3.30% (20% TCP), 4.65% (40% TCP), and 3.67% (60% TCP). The E-beam samples generally had more volume reduction in all experimental groups. Therefore, E-beam treatment may accelerate degradation. In our live micro-CT analyses, most volume reduction arose in the first four weeks after implantation and slowed between 4 and 20 weeks in all groups. E-beam groups showed greater volume reduction at every time point, which is consistent with the results by micro-CT analysis. Histology results suggest the biocompatibility of TCP/PCL composite filaments.https://www.mdpi.com/2072-666X/11/3/2733d printingβ-tricalcium phosphate/polycaprolactone (β-tcp/pcl) compositebone tissue engineeringelectron beam sterilization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jin-Ho Kang Janelle Kaneda Jae-Gon Jang Kumaresan Sakthiabirami Elaine Lui Carolyn Kim Aijun Wang Sang-Won Park Yunzhi Peter Yang |
spellingShingle |
Jin-Ho Kang Janelle Kaneda Jae-Gon Jang Kumaresan Sakthiabirami Elaine Lui Carolyn Kim Aijun Wang Sang-Won Park Yunzhi Peter Yang The Influence of Electron Beam Sterilization on In Vivo Degradation of β-TCP/PCL of Different Composite Ratios for Bone Tissue Engineering Micromachines 3d printing β-tricalcium phosphate/polycaprolactone (β-tcp/pcl) composite bone tissue engineering electron beam sterilization |
author_facet |
Jin-Ho Kang Janelle Kaneda Jae-Gon Jang Kumaresan Sakthiabirami Elaine Lui Carolyn Kim Aijun Wang Sang-Won Park Yunzhi Peter Yang |
author_sort |
Jin-Ho Kang |
title |
The Influence of Electron Beam Sterilization on In Vivo Degradation of β-TCP/PCL of Different Composite Ratios for Bone Tissue Engineering |
title_short |
The Influence of Electron Beam Sterilization on In Vivo Degradation of β-TCP/PCL of Different Composite Ratios for Bone Tissue Engineering |
title_full |
The Influence of Electron Beam Sterilization on In Vivo Degradation of β-TCP/PCL of Different Composite Ratios for Bone Tissue Engineering |
title_fullStr |
The Influence of Electron Beam Sterilization on In Vivo Degradation of β-TCP/PCL of Different Composite Ratios for Bone Tissue Engineering |
title_full_unstemmed |
The Influence of Electron Beam Sterilization on In Vivo Degradation of β-TCP/PCL of Different Composite Ratios for Bone Tissue Engineering |
title_sort |
influence of electron beam sterilization on in vivo degradation of β-tcp/pcl of different composite ratios for bone tissue engineering |
publisher |
MDPI AG |
series |
Micromachines |
issn |
2072-666X |
publishDate |
2020-03-01 |
description |
We evaluated the effect of electron beam (E-beam) sterilization (25 kGy, ISO 11137) on the degradation of β-tricalcium phosphate/polycaprolactone (β-TCP/PCL) composite filaments of various ratios (0:100, 20:80, 40:60, and 60:40 TCP:PCL by mass) in a rat subcutaneous model for 24 weeks. Volumes of the samples before implantation and after explantation were measured using micro-computed tomography (micro-CT). The filament volume changes before sacrifice were also measured using a live micro-CT. In our micro-CT analyses, there was no significant difference in volume change between the E-beam treated groups and non-E-beam treated groups of the same β-TCP to PCL ratios, except for the 0% β-TCP group. However, the average volume reduction differences between the E-beam and non-E-beam groups in the same-ratio samples were 0.76% (0% TCP), 3.30% (20% TCP), 4.65% (40% TCP), and 3.67% (60% TCP). The E-beam samples generally had more volume reduction in all experimental groups. Therefore, E-beam treatment may accelerate degradation. In our live micro-CT analyses, most volume reduction arose in the first four weeks after implantation and slowed between 4 and 20 weeks in all groups. E-beam groups showed greater volume reduction at every time point, which is consistent with the results by micro-CT analysis. Histology results suggest the biocompatibility of TCP/PCL composite filaments. |
topic |
3d printing β-tricalcium phosphate/polycaprolactone (β-tcp/pcl) composite bone tissue engineering electron beam sterilization |
url |
https://www.mdpi.com/2072-666X/11/3/273 |
work_keys_str_mv |
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