The Influence of Electron Beam Sterilization on In Vivo Degradation of β-TCP/PCL of Different Composite Ratios for Bone Tissue Engineering

We evaluated the effect of electron beam (E-beam) sterilization (25 kGy, ISO 11137) on the degradation of β-tricalcium phosphate/polycaprolactone (β-TCP/PCL) composite filaments of various ratios (0:100, 20:80, 40:60, and 60:40 TCP:PCL by mass) in a rat subcutaneous model for 24 we...

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Main Authors: Jin-Ho Kang, Janelle Kaneda, Jae-Gon Jang, Kumaresan Sakthiabirami, Elaine Lui, Carolyn Kim, Aijun Wang, Sang-Won Park, Yunzhi Peter Yang
Format: Article
Language:English
Published: MDPI AG 2020-03-01
Series:Micromachines
Subjects:
Online Access:https://www.mdpi.com/2072-666X/11/3/273
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spelling doaj-7570a30b672549e6b4cb19c1b653b4962020-11-25T02:15:07ZengMDPI AGMicromachines2072-666X2020-03-0111327310.3390/mi11030273mi11030273The Influence of Electron Beam Sterilization on In Vivo Degradation of β-TCP/PCL of Different Composite Ratios for Bone Tissue EngineeringJin-Ho Kang0Janelle Kaneda1Jae-Gon Jang2Kumaresan Sakthiabirami3Elaine Lui4Carolyn Kim5Aijun Wang6Sang-Won Park7Yunzhi Peter Yang8Department of Prosthodontics, School of Dentistry, Chonnam National University, Gwanju 61186, KoreaDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USADepartment of Prosthodontics, School of Dentistry, Chonnam National University, Gwanju 61186, KoreaDepartment of Prosthodontics, School of Dentistry, Chonnam National University, Gwanju 61186, KoreaDepartment of Mechanical Engineering, Stanford University, Stanford, CA 94305, USADepartment of Mechanical Engineering, Stanford University, Stanford, CA 94305, USASurgical Bioengineering Laboratory, Department of Surgery, School of Medicine, University of California–Davis, Sacramento, CA 95817, USADepartment of Prosthodontics, School of Dentistry, Chonnam National University, Gwanju 61186, KoreaDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USAWe evaluated the effect of electron beam (E-beam) sterilization (25 kGy, ISO 11137) on the degradation of β-tricalcium phosphate/polycaprolactone (β-TCP/PCL) composite filaments of various ratios (0:100, 20:80, 40:60, and 60:40 TCP:PCL by mass) in a rat subcutaneous model for 24 weeks. Volumes of the samples before implantation and after explantation were measured using micro-computed tomography (micro-CT). The filament volume changes before sacrifice were also measured using a live micro-CT. In our micro-CT analyses, there was no significant difference in volume change between the E-beam treated groups and non-E-beam treated groups of the same β-TCP to PCL ratios, except for the 0% β-TCP group. However, the average volume reduction differences between the E-beam and non-E-beam groups in the same-ratio samples were 0.76% (0% TCP), 3.30% (20% TCP), 4.65% (40% TCP), and 3.67% (60% TCP). The E-beam samples generally had more volume reduction in all experimental groups. Therefore, E-beam treatment may accelerate degradation. In our live micro-CT analyses, most volume reduction arose in the first four weeks after implantation and slowed between 4 and 20 weeks in all groups. E-beam groups showed greater volume reduction at every time point, which is consistent with the results by micro-CT analysis. Histology results suggest the biocompatibility of TCP/PCL composite filaments.https://www.mdpi.com/2072-666X/11/3/2733d printingβ-tricalcium phosphate/polycaprolactone (β-tcp/pcl) compositebone tissue engineeringelectron beam sterilization
collection DOAJ
language English
format Article
sources DOAJ
author Jin-Ho Kang
Janelle Kaneda
Jae-Gon Jang
Kumaresan Sakthiabirami
Elaine Lui
Carolyn Kim
Aijun Wang
Sang-Won Park
Yunzhi Peter Yang
spellingShingle Jin-Ho Kang
Janelle Kaneda
Jae-Gon Jang
Kumaresan Sakthiabirami
Elaine Lui
Carolyn Kim
Aijun Wang
Sang-Won Park
Yunzhi Peter Yang
The Influence of Electron Beam Sterilization on In Vivo Degradation of β-TCP/PCL of Different Composite Ratios for Bone Tissue Engineering
Micromachines
3d printing
β-tricalcium phosphate/polycaprolactone (β-tcp/pcl) composite
bone tissue engineering
electron beam sterilization
author_facet Jin-Ho Kang
Janelle Kaneda
Jae-Gon Jang
Kumaresan Sakthiabirami
Elaine Lui
Carolyn Kim
Aijun Wang
Sang-Won Park
Yunzhi Peter Yang
author_sort Jin-Ho Kang
title The Influence of Electron Beam Sterilization on In Vivo Degradation of β-TCP/PCL of Different Composite Ratios for Bone Tissue Engineering
title_short The Influence of Electron Beam Sterilization on In Vivo Degradation of β-TCP/PCL of Different Composite Ratios for Bone Tissue Engineering
title_full The Influence of Electron Beam Sterilization on In Vivo Degradation of β-TCP/PCL of Different Composite Ratios for Bone Tissue Engineering
title_fullStr The Influence of Electron Beam Sterilization on In Vivo Degradation of β-TCP/PCL of Different Composite Ratios for Bone Tissue Engineering
title_full_unstemmed The Influence of Electron Beam Sterilization on In Vivo Degradation of β-TCP/PCL of Different Composite Ratios for Bone Tissue Engineering
title_sort influence of electron beam sterilization on in vivo degradation of β-tcp/pcl of different composite ratios for bone tissue engineering
publisher MDPI AG
series Micromachines
issn 2072-666X
publishDate 2020-03-01
description We evaluated the effect of electron beam (E-beam) sterilization (25 kGy, ISO 11137) on the degradation of β-tricalcium phosphate/polycaprolactone (β-TCP/PCL) composite filaments of various ratios (0:100, 20:80, 40:60, and 60:40 TCP:PCL by mass) in a rat subcutaneous model for 24 weeks. Volumes of the samples before implantation and after explantation were measured using micro-computed tomography (micro-CT). The filament volume changes before sacrifice were also measured using a live micro-CT. In our micro-CT analyses, there was no significant difference in volume change between the E-beam treated groups and non-E-beam treated groups of the same β-TCP to PCL ratios, except for the 0% β-TCP group. However, the average volume reduction differences between the E-beam and non-E-beam groups in the same-ratio samples were 0.76% (0% TCP), 3.30% (20% TCP), 4.65% (40% TCP), and 3.67% (60% TCP). The E-beam samples generally had more volume reduction in all experimental groups. Therefore, E-beam treatment may accelerate degradation. In our live micro-CT analyses, most volume reduction arose in the first four weeks after implantation and slowed between 4 and 20 weeks in all groups. E-beam groups showed greater volume reduction at every time point, which is consistent with the results by micro-CT analysis. Histology results suggest the biocompatibility of TCP/PCL composite filaments.
topic 3d printing
β-tricalcium phosphate/polycaprolactone (β-tcp/pcl) composite
bone tissue engineering
electron beam sterilization
url https://www.mdpi.com/2072-666X/11/3/273
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