Mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in human astrocytoma cells (CCF-STTG1).

Lactate has long been regarded as an end product of anaerobic energy production and its fate in cerebral metabolism has not been precisely delineated. In this report, we demonstrate, for the first time, the ability of a human astrocytic cell line (CCF-STTG1) to consume lactate and to generate ATP vi...

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Main Authors: Joseph Lemire, Ryan J Mailloux, Vasu D Appanna
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2008-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2212712?pdf=render
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spelling doaj-7527ad9ffca148b1aca4a8ea09db64112020-11-25T01:53:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-0132e155010.1371/journal.pone.0001550Mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in human astrocytoma cells (CCF-STTG1).Joseph LemireRyan J MaillouxVasu D AppannaLactate has long been regarded as an end product of anaerobic energy production and its fate in cerebral metabolism has not been precisely delineated. In this report, we demonstrate, for the first time, the ability of a human astrocytic cell line (CCF-STTG1) to consume lactate and to generate ATP via oxidative phosphorylation. (13)C-NMR and HPLC analyses aided in the identification of tricarboxylic acid (TCA) cyle metabolites and ATP in the astrocytic mitochondria incubated with lactate. Oxamate, an inhibitor of lactate dehydrogenase (LDH), abolished mitochondrial lactate consumption. Electrophoretic and fluorescence microscopic analyses helped localize LDH in the mitochondria. Taken together, this study implicates lactate as an important contributor to ATP metabolism in the brain, a finding that may significantly change our notion of how this important organ manipulates its energy budget.http://europepmc.org/articles/PMC2212712?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Joseph Lemire
Ryan J Mailloux
Vasu D Appanna
spellingShingle Joseph Lemire
Ryan J Mailloux
Vasu D Appanna
Mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in human astrocytoma cells (CCF-STTG1).
PLoS ONE
author_facet Joseph Lemire
Ryan J Mailloux
Vasu D Appanna
author_sort Joseph Lemire
title Mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in human astrocytoma cells (CCF-STTG1).
title_short Mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in human astrocytoma cells (CCF-STTG1).
title_full Mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in human astrocytoma cells (CCF-STTG1).
title_fullStr Mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in human astrocytoma cells (CCF-STTG1).
title_full_unstemmed Mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in human astrocytoma cells (CCF-STTG1).
title_sort mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in human astrocytoma cells (ccf-sttg1).
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2008-01-01
description Lactate has long been regarded as an end product of anaerobic energy production and its fate in cerebral metabolism has not been precisely delineated. In this report, we demonstrate, for the first time, the ability of a human astrocytic cell line (CCF-STTG1) to consume lactate and to generate ATP via oxidative phosphorylation. (13)C-NMR and HPLC analyses aided in the identification of tricarboxylic acid (TCA) cyle metabolites and ATP in the astrocytic mitochondria incubated with lactate. Oxamate, an inhibitor of lactate dehydrogenase (LDH), abolished mitochondrial lactate consumption. Electrophoretic and fluorescence microscopic analyses helped localize LDH in the mitochondria. Taken together, this study implicates lactate as an important contributor to ATP metabolism in the brain, a finding that may significantly change our notion of how this important organ manipulates its energy budget.
url http://europepmc.org/articles/PMC2212712?pdf=render
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AT ryanjmailloux mitochondriallactatedehydrogenaseisinvolvedinoxidativeenergymetabolisminhumanastrocytomacellsccfsttg1
AT vasudappanna mitochondriallactatedehydrogenaseisinvolvedinoxidativeenergymetabolisminhumanastrocytomacellsccfsttg1
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