Universal Molecular Triggers of Stress Responses in Cyanobacterium <i>Synechocystis</i>

Systemic analysis of stress-induced transcription in the cyanobacterium <i>Synechocystis</i> sp. strain PCC 6803 identifies a number of genes as being induced in response to most abiotic stressors (heat, osmotic, saline, acid stress, strong light, and ultraviolet radiation). Genes for he...

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Main Authors: Kirill S. Mironov, Maria A. Sinetova, Maria Shumskaya, Dmitry A. Los
Format: Article
Language:English
Published: MDPI AG 2019-08-01
Series:Life
Subjects:
ROS
Online Access:https://www.mdpi.com/2075-1729/9/3/67
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spelling doaj-74fa7cf04904415bb3e36f1c2e09fea92020-11-25T02:16:12ZengMDPI AGLife2075-17292019-08-01936710.3390/life9030067life9030067Universal Molecular Triggers of Stress Responses in Cyanobacterium <i>Synechocystis</i>Kirill S. Mironov0Maria A. Sinetova1Maria Shumskaya2Dmitry A. Los3Department of Molecular Biosystems, K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya street 35, 127276 Moscow, RussianDepartment of Molecular Biosystems, K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya street 35, 127276 Moscow, RussianDepartment of Biology, School of Natural Sciences, Kean University, 1000 Morris Ave, Union, NJ 07083, USADepartment of Molecular Biosystems, K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya street 35, 127276 Moscow, RussianSystemic analysis of stress-induced transcription in the cyanobacterium <i>Synechocystis</i> sp. strain PCC 6803 identifies a number of genes as being induced in response to most abiotic stressors (heat, osmotic, saline, acid stress, strong light, and ultraviolet radiation). Genes for heat-shock proteins (HSPs) are activated by all these stresses and form a group that universally responds to all environmental changes. The functions of universal triggers of stress responses in cyanobacteria can be performed by reactive oxygen species (ROS), in particular H<sub>2</sub>O<sub>2</sub>, as well as changes in the redox potential of the components of the photosynthetic electron transport chain. The double mutant of <i>Synechocystis</i> sp. PCC 6803 (<i>katG/tpx</i>, or <i>sll1987/sll0755</i>), which is defective in antioxidant enzymes catalase (KatG) and thioredoxin peroxidase (Tpx), cannot grow in the presence of exogenous hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>); and it is extremely sensitive to low concentrations of H<sub>2</sub>O<sub>2</sub>, especially under conditions of cold stress. Experiments on this mutant demonstrate that H<sub>2</sub>O<sub>2</sub> is involved in regulation of gene expression that responds to a decrease in ambient temperature, and affects both the perception and the signal transduction of cold stress. In addition, they suggest that formation of ROS largely depends on the physical state of the membranes such as fluidity or viscosity. In cyanobacteria, an increase in membrane turnover leads to a decrease in the formation of ROS and an increase in resistance to cold stress. Therefore: (1) H<sub>2</sub>O<sub>2</sub> is the universal trigger of stress responses in cyanobacterial cells; (2) ROS formation (in particular, H<sub>2</sub>O<sub>2</sub>) depends on the physical properties of both cytoplasmic and thylakoid membranes; (3) The destructive effect of H<sub>2</sub>O<sub>2</sub> is reduced by increasing of fluidity of biological membranes.https://www.mdpi.com/2075-1729/9/3/67cyanobacteriamembrane fluidityROSstress responsesuniversal triggers
collection DOAJ
language English
format Article
sources DOAJ
author Kirill S. Mironov
Maria A. Sinetova
Maria Shumskaya
Dmitry A. Los
spellingShingle Kirill S. Mironov
Maria A. Sinetova
Maria Shumskaya
Dmitry A. Los
Universal Molecular Triggers of Stress Responses in Cyanobacterium <i>Synechocystis</i>
Life
cyanobacteria
membrane fluidity
ROS
stress responses
universal triggers
author_facet Kirill S. Mironov
Maria A. Sinetova
Maria Shumskaya
Dmitry A. Los
author_sort Kirill S. Mironov
title Universal Molecular Triggers of Stress Responses in Cyanobacterium <i>Synechocystis</i>
title_short Universal Molecular Triggers of Stress Responses in Cyanobacterium <i>Synechocystis</i>
title_full Universal Molecular Triggers of Stress Responses in Cyanobacterium <i>Synechocystis</i>
title_fullStr Universal Molecular Triggers of Stress Responses in Cyanobacterium <i>Synechocystis</i>
title_full_unstemmed Universal Molecular Triggers of Stress Responses in Cyanobacterium <i>Synechocystis</i>
title_sort universal molecular triggers of stress responses in cyanobacterium <i>synechocystis</i>
publisher MDPI AG
series Life
issn 2075-1729
publishDate 2019-08-01
description Systemic analysis of stress-induced transcription in the cyanobacterium <i>Synechocystis</i> sp. strain PCC 6803 identifies a number of genes as being induced in response to most abiotic stressors (heat, osmotic, saline, acid stress, strong light, and ultraviolet radiation). Genes for heat-shock proteins (HSPs) are activated by all these stresses and form a group that universally responds to all environmental changes. The functions of universal triggers of stress responses in cyanobacteria can be performed by reactive oxygen species (ROS), in particular H<sub>2</sub>O<sub>2</sub>, as well as changes in the redox potential of the components of the photosynthetic electron transport chain. The double mutant of <i>Synechocystis</i> sp. PCC 6803 (<i>katG/tpx</i>, or <i>sll1987/sll0755</i>), which is defective in antioxidant enzymes catalase (KatG) and thioredoxin peroxidase (Tpx), cannot grow in the presence of exogenous hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>); and it is extremely sensitive to low concentrations of H<sub>2</sub>O<sub>2</sub>, especially under conditions of cold stress. Experiments on this mutant demonstrate that H<sub>2</sub>O<sub>2</sub> is involved in regulation of gene expression that responds to a decrease in ambient temperature, and affects both the perception and the signal transduction of cold stress. In addition, they suggest that formation of ROS largely depends on the physical state of the membranes such as fluidity or viscosity. In cyanobacteria, an increase in membrane turnover leads to a decrease in the formation of ROS and an increase in resistance to cold stress. Therefore: (1) H<sub>2</sub>O<sub>2</sub> is the universal trigger of stress responses in cyanobacterial cells; (2) ROS formation (in particular, H<sub>2</sub>O<sub>2</sub>) depends on the physical properties of both cytoplasmic and thylakoid membranes; (3) The destructive effect of H<sub>2</sub>O<sub>2</sub> is reduced by increasing of fluidity of biological membranes.
topic cyanobacteria
membrane fluidity
ROS
stress responses
universal triggers
url https://www.mdpi.com/2075-1729/9/3/67
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