Reliable and Sensitive Nested PCR for the Detection of <i>Chlamydia</i> in Sputum

Reliable and Sensitive Nested PCR for the Detection of <i>Chlamydia</i> in Sputum

Chlamydia are Gram-negative, intracellular pathogens colonizing epithelial mucosa. They cause primarily atypical pneumonia and have recently been associated with chronic diseases. Diagnostics relies almost exclusively on serological methods; PCR tests are used rarely because in patients with positiv...

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Bibliographic Details
Main Authors: Martina Smolejová, Iveta Cihová, Pavol Sulo
Format: Article
Language:English
Published: MDPI AG 2021-04-01
Series:Microorganisms
Subjects:
<i>Chlamydia</i>
diagnostics
nested PCR
zoonosis
DNA sequence
detection limit
Online Access:https://www.mdpi.com/2076-2607/9/5/935

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