• Main Authors: S. Christobher, M. Periyasamy, H.E. Syed Mohamed, A. Sadiq Bukhari, Alagamuthu Karthickkumar, Vellingiri Balachandar
• Format: Article
• Language: English
• Published: SpringerOpen 2017-04-01
• Series: Egyptian Journal of Medical Human Genetics
• Subjects: Chromatid aberration; Micronuclei; Comet assay; Lymphocytes
• Online Access: http://www.sciencedirect.com/science/article/pii/S1110863016300313

Background: Tobacco smoke causes serious health ill effects to human population. Cancer and cardiovascular diseases are more common in smoking subjects. Aim: The present study is focused about the genetical changes in smoking subjects based on their age and pack years. Subjects and methods: Based on a survey report, 160 subjects are selected from Tiruchirappalli district, Tamil Nadu, India. Venous blood and buccal smear samples are collected from each subject. Results: Increased CA is observed in heavy smokers compared to light and non smokers which is 8.90 ± 2.58, 4.58 ± 2.36 and 4.31 ± 1.17, respectively. Both medium and light smokers showed significantly increased CA frequencies than control. Comet assay showed increased percentage of abnormalities in smokers (light, medium and heavy) than non-smokers. Conclusion: The frequencies of MN in buccal epithelial and blood lymphocytes are high in smokers; particularly heavy smoker group showed significantly increased results. Among them, the lymphocytic cells showed high MN frequency.