Fed-Batch Production of Saccharomyces cerevisiae L-Asparaginase II by Recombinant Pichia pastoris MUTs Strain

Fed-Batch Production of Saccharomyces cerevisiae L-Asparaginase II by Recombinant Pichia pastoris MUTs Strain

L-Asparaginase (ASNase) is used in the treatment of acute lymphoblastic leukemia, being produced and commercialized only from bacterial sources. Alternative Saccharomyces cerevisiae ASNase II coded by the ASP3 gene was biosynthesized by recombinant Pichia pastoris MUTs under the control of the AOX1...

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Bibliographic Details
Main Authors: David Rodrigues, Omar Pillaca-Pullo, Karin Torres-Obreque, Juan Flores-Santos, Ignacio Sánchez-Moguel, Marcela V. Pimenta, Tajindar Basi, Attilio Converti, André M. Lopes, Gisele Monteiro, Luís P. Fonseca, Adalberto Jr. Pessoa
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-02-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
L-Asparaginase
Pichia pastoris
defined medium
fed-batch fermentation
high cell density culture
heterologous protein production
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2019.00016/full

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