Basic fibroblast growth factor and agarose gel promote the ability of immune privilege of allogeneic cartilage transplantation in rats
Objective: Allogeneic cartilage transplantation is used to treat severe osteochondral defects or cartilaginous injury. However, acute immune rejection has been a key problem interfering with graft healing. Methods: Full-thickness osteochondral defects were performed in Sprague Dawley rats. The allog...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-05-01
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| Series: | Journal of Orthopaedic Translation |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214031X19300488 |
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doaj-74960dd8dc534ca7b36fda9917d12e57 |
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Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Fan Yang Yu Zhang Baoyi Liu Meng Cao Jiahui Yang Fengde Tian Pei Yang Kairong Qin Dewei Zhao |
| spellingShingle |
Fan Yang Yu Zhang Baoyi Liu Meng Cao Jiahui Yang Fengde Tian Pei Yang Kairong Qin Dewei Zhao Basic fibroblast growth factor and agarose gel promote the ability of immune privilege of allogeneic cartilage transplantation in rats Journal of Orthopaedic Translation Cartilage transplantation Immune privilege Inflammatory factors |
| author_facet |
Fan Yang Yu Zhang Baoyi Liu Meng Cao Jiahui Yang Fengde Tian Pei Yang Kairong Qin Dewei Zhao |
| author_sort |
Fan Yang |
| title |
Basic fibroblast growth factor and agarose gel promote the ability of immune privilege of allogeneic cartilage transplantation in rats |
| title_short |
Basic fibroblast growth factor and agarose gel promote the ability of immune privilege of allogeneic cartilage transplantation in rats |
| title_full |
Basic fibroblast growth factor and agarose gel promote the ability of immune privilege of allogeneic cartilage transplantation in rats |
| title_fullStr |
Basic fibroblast growth factor and agarose gel promote the ability of immune privilege of allogeneic cartilage transplantation in rats |
| title_full_unstemmed |
Basic fibroblast growth factor and agarose gel promote the ability of immune privilege of allogeneic cartilage transplantation in rats |
| title_sort |
basic fibroblast growth factor and agarose gel promote the ability of immune privilege of allogeneic cartilage transplantation in rats |
| publisher |
Elsevier |
| series |
Journal of Orthopaedic Translation |
| issn |
2214-031X |
| publishDate |
2020-05-01 |
| description |
Objective: Allogeneic cartilage transplantation is used to treat severe osteochondral defects or cartilaginous injury. However, acute immune rejection has been a key problem interfering with graft healing. Methods: Full-thickness osteochondral defects were performed in Sprague Dawley rats. The allograft implants were set into the defect region. Blood and spleen samples from Postoperative Day 3 onward were collected for inflammatory cell analysis, including analysis of monocytes, natural killer cells, CD4+CD25+Foxp3+ regulatory T cells, CD4+ T cells, and CD8+ T cells. Gross observation and histologic staining (hematoxylin and eosin, toluidine blue) were carried out at the same time point to assess the repair effect of the cartilage graft and the degree of immune rejection. Results: Treatment with basic fibroblast growth factor, agarose gel, and allogeneic cartilage was similar to that of the autologous group. The percentage of monocytes in allografts was at a higher level in the spleen and blood; the frequency of CD4+ T cells in the allogeneic group was higher than in the autologous group and the other agarose groups at 6 weeks after transplantation. The number of regulatory T cells in the autograft was increased from Postoperative Week 1; similar results were observed in groups containing basic fibroblast growth factor beginning at Postoperative Week 3. Conclusions: Allogeneic cartilage transplantation induces acute immune rejection, which compromises the validity of the implant. The combination of basic fibroblast growth factor and agarose gel facilitates the goal of immune privilege and promotes the success of the allograft tissues. The translational potential of this article: This study investigated the combination of basic fibroblast growth factor (bFGF) and agarose gel facilitates promotes the success of the allograft tissues transplantation. This work may help clinicians find a new way to repair articular cartilage damage. This will affect the treatment of articular cartilage movement injuries and arthritis. |
| topic |
Cartilage transplantation Immune privilege Inflammatory factors |
| url |
http://www.sciencedirect.com/science/article/pii/S2214031X19300488 |
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1724604309017985024 |
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doaj-74960dd8dc534ca7b36fda9917d12e572020-11-25T03:23:49ZengElsevierJournal of Orthopaedic Translation2214-031X2020-05-01227380Basic fibroblast growth factor and agarose gel promote the ability of immune privilege of allogeneic cartilage transplantation in ratsFan Yang0Yu Zhang1Baoyi Liu2Meng Cao3Jiahui Yang4Fengde Tian5Pei Yang6Kairong Qin7Dewei Zhao8Department of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Linggong Road, Dalian, Liaoning, China; Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, No 6 Jiefang Street, Dalian, Liaoning, ChinaDepartment of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, No 6 Jiefang Street, Dalian, Liaoning, ChinaDepartment of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, No 6 Jiefang Street, Dalian, Liaoning, China; Postdoctoral Workstation, Affiliated Zhongshan Hospital of Dalian University, No 6 Jiefang Street, Dalian, Liaoning, China; Department of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Linggong Road, Dalian, Liaoning, China.Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, No 6 Jiefang Street, Dalian, Liaoning, ChinaDepartment of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, No 6 Jiefang Street, Dalian, Liaoning, ChinaDepartment of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Linggong Road, Dalian, Liaoning, China; Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, No 6 Jiefang Street, Dalian, Liaoning, ChinaThe First Department of Orthopaedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shanxi, 710000, ChinaDepartment of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Linggong Road, Dalian, Liaoning, ChinaDepartment of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Linggong Road, Dalian, Liaoning, China; Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, No 6 Jiefang Street, Dalian, Liaoning, China; Corresponding author. Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, No 6 Jiefang Street, Zhongshan District, Dalian, Liaoning 116001, China.Objective: Allogeneic cartilage transplantation is used to treat severe osteochondral defects or cartilaginous injury. However, acute immune rejection has been a key problem interfering with graft healing. Methods: Full-thickness osteochondral defects were performed in Sprague Dawley rats. The allograft implants were set into the defect region. Blood and spleen samples from Postoperative Day 3 onward were collected for inflammatory cell analysis, including analysis of monocytes, natural killer cells, CD4+CD25+Foxp3+ regulatory T cells, CD4+ T cells, and CD8+ T cells. Gross observation and histologic staining (hematoxylin and eosin, toluidine blue) were carried out at the same time point to assess the repair effect of the cartilage graft and the degree of immune rejection. Results: Treatment with basic fibroblast growth factor, agarose gel, and allogeneic cartilage was similar to that of the autologous group. The percentage of monocytes in allografts was at a higher level in the spleen and blood; the frequency of CD4+ T cells in the allogeneic group was higher than in the autologous group and the other agarose groups at 6 weeks after transplantation. The number of regulatory T cells in the autograft was increased from Postoperative Week 1; similar results were observed in groups containing basic fibroblast growth factor beginning at Postoperative Week 3. Conclusions: Allogeneic cartilage transplantation induces acute immune rejection, which compromises the validity of the implant. The combination of basic fibroblast growth factor and agarose gel facilitates the goal of immune privilege and promotes the success of the allograft tissues. The translational potential of this article: This study investigated the combination of basic fibroblast growth factor (bFGF) and agarose gel facilitates promotes the success of the allograft tissues transplantation. This work may help clinicians find a new way to repair articular cartilage damage. This will affect the treatment of articular cartilage movement injuries and arthritis.http://www.sciencedirect.com/science/article/pii/S2214031X19300488Cartilage transplantationImmune privilegeInflammatory factors |