YALI0C22088g from Yarrowia lipolytica catalyses the conversion of l‐methionine into volatile organic sulfur‐containing compounds

Summary The enzymatic conversion of l‐methionine (l‐Met) into volatile organic sulfur‐containing compounds (VOSCs) plays an important role in developing the characteristic aroma of foods. However, the mechanism for the direct conversion of l‐Met into VOSCs is still unclear in yeast cells used to mak...

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Main Authors: Quan‐Lu Zhao, Zhu‐Lin Wang, Lan Yang, Sai Zhang, Kai‐Zhi Jia
Format: Article
Language:English
Published: Wiley 2021-07-01
Series:Microbial Biotechnology
Online Access:https://doi.org/10.1111/1751-7915.13796
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spelling doaj-747162c1e58e43069de3afbd85bde51d2021-07-26T21:47:23ZengWileyMicrobial Biotechnology1751-79152021-07-011441462147110.1111/1751-7915.13796YALI0C22088g from Yarrowia lipolytica catalyses the conversion of l‐methionine into volatile organic sulfur‐containing compoundsQuan‐Lu Zhao0Zhu‐Lin Wang1Lan Yang2Sai Zhang3Kai‐Zhi Jia4Key Laboratory of Fermentation Engineering (Ministry of Education) Hubei Key Laboratory of Industrial Microbiology Hubei Provincial Cooperative Innovation Center of Industrial Fermentation Hubei University of Technology Wuhan 430068 ChinaKey Laboratory of Fermentation Engineering (Ministry of Education) Hubei Key Laboratory of Industrial Microbiology Hubei Provincial Cooperative Innovation Center of Industrial Fermentation Hubei University of Technology Wuhan 430068 ChinaKey Laboratory of Fermentation Engineering (Ministry of Education) Hubei Key Laboratory of Industrial Microbiology Hubei Provincial Cooperative Innovation Center of Industrial Fermentation Hubei University of Technology Wuhan 430068 ChinaKey Laboratory of Fermentation Engineering (Ministry of Education) Hubei Key Laboratory of Industrial Microbiology Hubei Provincial Cooperative Innovation Center of Industrial Fermentation Hubei University of Technology Wuhan 430068 ChinaKey Laboratory of Fermentation Engineering (Ministry of Education) Hubei Key Laboratory of Industrial Microbiology Hubei Provincial Cooperative Innovation Center of Industrial Fermentation Hubei University of Technology Wuhan 430068 ChinaSummary The enzymatic conversion of l‐methionine (l‐Met) into volatile organic sulfur‐containing compounds (VOSCs) plays an important role in developing the characteristic aroma of foods. However, the mechanism for the direct conversion of l‐Met into VOSCs is still unclear in yeast cells used to make food products. Here, we show that the transcription profile of YALI0C22088g from Yarrowia lipolytica correlates positively with l‐Met addition. YALI0C22088g catalyses the γ‐elimination of l‐Met, directly converting l‐Met into VOSCs. YALI0C22088g also exhibits strong C‐S lysis activities towards l‐cystathionine and the other sulfur‐containing compounds and forms a distinct cystathionine‐γ‐lyase subgroup. We identified eight key amino acid residues in YALI0C22088g, and we inferred that the size of the tunnel and the charges carried by the entrance amino acid residue are the determinants for the enzymatic conversion of l‐Met into VOSCs. These findings reveal the formation mechanism of VOSCs produced directly from l‐Met via the demethiolation pathway in Yarrowia lipolytica, which provides a rationale for engineering the enzymatic conversion of l‐Met into VOSCs and thus stimulates the enzymatic production of aroma compounds.https://doi.org/10.1111/1751-7915.13796
collection DOAJ
language English
format Article
sources DOAJ
author Quan‐Lu Zhao
Zhu‐Lin Wang
Lan Yang
Sai Zhang
Kai‐Zhi Jia
spellingShingle Quan‐Lu Zhao
Zhu‐Lin Wang
Lan Yang
Sai Zhang
Kai‐Zhi Jia
YALI0C22088g from Yarrowia lipolytica catalyses the conversion of l‐methionine into volatile organic sulfur‐containing compounds
Microbial Biotechnology
author_facet Quan‐Lu Zhao
Zhu‐Lin Wang
Lan Yang
Sai Zhang
Kai‐Zhi Jia
author_sort Quan‐Lu Zhao
title YALI0C22088g from Yarrowia lipolytica catalyses the conversion of l‐methionine into volatile organic sulfur‐containing compounds
title_short YALI0C22088g from Yarrowia lipolytica catalyses the conversion of l‐methionine into volatile organic sulfur‐containing compounds
title_full YALI0C22088g from Yarrowia lipolytica catalyses the conversion of l‐methionine into volatile organic sulfur‐containing compounds
title_fullStr YALI0C22088g from Yarrowia lipolytica catalyses the conversion of l‐methionine into volatile organic sulfur‐containing compounds
title_full_unstemmed YALI0C22088g from Yarrowia lipolytica catalyses the conversion of l‐methionine into volatile organic sulfur‐containing compounds
title_sort yali0c22088g from yarrowia lipolytica catalyses the conversion of l‐methionine into volatile organic sulfur‐containing compounds
publisher Wiley
series Microbial Biotechnology
issn 1751-7915
publishDate 2021-07-01
description Summary The enzymatic conversion of l‐methionine (l‐Met) into volatile organic sulfur‐containing compounds (VOSCs) plays an important role in developing the characteristic aroma of foods. However, the mechanism for the direct conversion of l‐Met into VOSCs is still unclear in yeast cells used to make food products. Here, we show that the transcription profile of YALI0C22088g from Yarrowia lipolytica correlates positively with l‐Met addition. YALI0C22088g catalyses the γ‐elimination of l‐Met, directly converting l‐Met into VOSCs. YALI0C22088g also exhibits strong C‐S lysis activities towards l‐cystathionine and the other sulfur‐containing compounds and forms a distinct cystathionine‐γ‐lyase subgroup. We identified eight key amino acid residues in YALI0C22088g, and we inferred that the size of the tunnel and the charges carried by the entrance amino acid residue are the determinants for the enzymatic conversion of l‐Met into VOSCs. These findings reveal the formation mechanism of VOSCs produced directly from l‐Met via the demethiolation pathway in Yarrowia lipolytica, which provides a rationale for engineering the enzymatic conversion of l‐Met into VOSCs and thus stimulates the enzymatic production of aroma compounds.
url https://doi.org/10.1111/1751-7915.13796
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