Generation of iPSC lines from peripheral blood mononuclear cells of identical twins both suffering from type 2 diabetes mellitus and one of them additionally diagnosed with atherosclerosis

Here we describe the generation of induced pluripotent stem cell (iPSC) lines from peripheral blood samples of identical twin sisters with type 2 diabetes mellitus (DM2). Two clonal lines from each patient (HU-DM2-A-1, HU-DM2-A-2 and HU-DM2-B-1, HU-DM2-B-2) were established via Sendai viral reprogra...

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Main Authors:	Eszter Szabó, Dóra Reé, Bálint Jezsó, Katalin Vincze, Gábor Földes, Andrea Á. Molnár, János M. Réthelyi, Ágota Apáti
Format:	Article
Language:	English
Published:	Elsevier 2020-12-01
Series:	Stem Cell Research
Online Access:	http://www.sciencedirect.com/science/article/pii/S1873506120303524

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spelling	doaj-7468e382acf0440782f7068f2dd470d32020-12-19T05:04:57ZengElsevierStem Cell Research1873-50612020-12-0149102051Generation of iPSC lines from peripheral blood mononuclear cells of identical twins both suffering from type 2 diabetes mellitus and one of them additionally diagnosed with atherosclerosisEszter Szabó0Dóra Reé1Bálint Jezsó2Katalin Vincze3Gábor Földes4Andrea Á. Molnár5János M. Réthelyi6Ágota Apáti7Institute of Enzymology, Research Center for Natural Sciences, Eötvös Loránd Research Network, HungaryInstitute of Enzymology, Research Center for Natural Sciences, Eötvös Loránd Research Network, HungaryInstitute of Enzymology, Research Center for Natural Sciences, Eötvös Loránd Research Network, HungaryInstitute of Enzymology, Research Center for Natural Sciences, Eötvös Loránd Research Network, Hungary; Molecular Psychiatry Research Group, National Brain Research Program (NAP), Hungarian Academy of Sciences and Semmelweis University, HungaryHeart and Vascular Center, Semmelweis University, Budapest, HungaryHeart and Vascular Center, Semmelweis University, Budapest, HungaryMolecular Psychiatry Research Group, National Brain Research Program (NAP), Hungarian Academy of Sciences and Semmelweis University, Hungary; Department of Psychiatry and Psychotherapy, Semmelweis University, Budapest, HungaryInstitute of Enzymology, Research Center for Natural Sciences, Eötvös Loránd Research Network, Hungary; Corresponding author.Here we describe the generation of induced pluripotent stem cell (iPSC) lines from peripheral blood samples of identical twin sisters with type 2 diabetes mellitus (DM2). Two clonal lines from each patient (HU-DM2-A-1, HU-DM2-A-2 and HU-DM2-B-1, HU-DM2-B-2) were established via Sendai viral reprograming of peripheral blood mononuclear cells, and characterized to confirm pluripotency and genetic integrity. The established iPSC lines can help to investigate DM2 related cellular phenotypes and provide a model system for drug testing.http://www.sciencedirect.com/science/article/pii/S1873506120303524
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Eszter Szabó Dóra Reé Bálint Jezsó Katalin Vincze Gábor Földes Andrea Á. Molnár János M. Réthelyi Ágota Apáti
spellingShingle	Eszter Szabó Dóra Reé Bálint Jezsó Katalin Vincze Gábor Földes Andrea Á. Molnár János M. Réthelyi Ágota Apáti Generation of iPSC lines from peripheral blood mononuclear cells of identical twins both suffering from type 2 diabetes mellitus and one of them additionally diagnosed with atherosclerosis Stem Cell Research
author_facet	Eszter Szabó Dóra Reé Bálint Jezsó Katalin Vincze Gábor Földes Andrea Á. Molnár János M. Réthelyi Ágota Apáti
author_sort	Eszter Szabó
title	Generation of iPSC lines from peripheral blood mononuclear cells of identical twins both suffering from type 2 diabetes mellitus and one of them additionally diagnosed with atherosclerosis
title_short	Generation of iPSC lines from peripheral blood mononuclear cells of identical twins both suffering from type 2 diabetes mellitus and one of them additionally diagnosed with atherosclerosis
title_full	Generation of iPSC lines from peripheral blood mononuclear cells of identical twins both suffering from type 2 diabetes mellitus and one of them additionally diagnosed with atherosclerosis
title_fullStr	Generation of iPSC lines from peripheral blood mononuclear cells of identical twins both suffering from type 2 diabetes mellitus and one of them additionally diagnosed with atherosclerosis
title_full_unstemmed	Generation of iPSC lines from peripheral blood mononuclear cells of identical twins both suffering from type 2 diabetes mellitus and one of them additionally diagnosed with atherosclerosis
title_sort	generation of ipsc lines from peripheral blood mononuclear cells of identical twins both suffering from type 2 diabetes mellitus and one of them additionally diagnosed with atherosclerosis
publisher	Elsevier
series	Stem Cell Research
issn	1873-5061
publishDate	2020-12-01
description	Here we describe the generation of induced pluripotent stem cell (iPSC) lines from peripheral blood samples of identical twin sisters with type 2 diabetes mellitus (DM2). Two clonal lines from each patient (HU-DM2-A-1, HU-DM2-A-2 and HU-DM2-B-1, HU-DM2-B-2) were established via Sendai viral reprograming of peripheral blood mononuclear cells, and characterized to confirm pluripotency and genetic integrity. The established iPSC lines can help to investigate DM2 related cellular phenotypes and provide a model system for drug testing.
url	http://www.sciencedirect.com/science/article/pii/S1873506120303524
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Generation of iPSC lines from peripheral blood mononuclear cells of identical twins both suffering from type 2 diabetes mellitus and one of them additionally diagnosed with atherosclerosis

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