Cloning of araA Gene Encoding L-Arabinose Isomerase from Marine Geobacillus stearothermophilus Isolated from Tanjung Api, Poso, Indonesia

Cloning of araA Gene Encoding L-Arabinose Isomerase from Marine Geobacillus stearothermophilus Isolated from Tanjung Api, Poso, Indonesia

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L-arabinose isomerase is an enzyme converting D-galactose to D-tagatose. D-tagatose is a potential sweetener-sucrose substitute which has low calorie. This research was to clone and sequence araA gene from marine bacterial strain Geobacillus stearothermophilus isolated from Tanjung Api Poso Indonesi...

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Bibliographic Details
Main Authors: DEWI FITRIANI, BUDI SAKSONO
Format: Article
Language:English
Published: Bogor Agricultural University 2010-06-01
Series:Hayati Journal of Biosciences
Subjects:
cloning
araA gene
marine bacterium
Geobacillus stearothermophilus
Online Access:http://journal.ipb.ac.id/index.php/hayati/article/view/1348/445
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spelling doaj-745e272e20764c7d8b5ed7eaee01930b2020-11-24T21:12:14ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942010-06-011725862Cloning of araA Gene Encoding L-Arabinose Isomerase from Marine Geobacillus stearothermophilus Isolated from Tanjung Api, Poso, IndonesiaDEWI FITRIANIBUDI SAKSONOL-arabinose isomerase is an enzyme converting D-galactose to D-tagatose. D-tagatose is a potential sweetener-sucrose substitute which has low calorie. This research was to clone and sequence araA gene from marine bacterial strain Geobacillus stearothermophilus isolated from Tanjung Api Poso Indonesia. The amplified araA gene consisted of 1494 bp nucleotides encoding 497 amino acids. DNA alignment analysis showed that the gene had high homology with that of G. stearothermophilus T6. The enzyme had optimum activity at high temperature and alkalin condition.http://journal.ipb.ac.id/index.php/hayati/article/view/1348/445cloningaraA genemarine bacteriumGeobacillus stearothermophilus
collection DOAJ
language English
format Article
sources DOAJ
author DEWI FITRIANI
BUDI SAKSONO
spellingShingle DEWI FITRIANI
BUDI SAKSONO
Cloning of araA Gene Encoding L-Arabinose Isomerase from Marine Geobacillus stearothermophilus Isolated from Tanjung Api, Poso, Indonesia
Hayati Journal of Biosciences
cloning
araA gene
marine bacterium
Geobacillus stearothermophilus
author_facet DEWI FITRIANI
BUDI SAKSONO
author_sort DEWI FITRIANI
title Cloning of araA Gene Encoding L-Arabinose Isomerase from Marine Geobacillus stearothermophilus Isolated from Tanjung Api, Poso, Indonesia
title_short Cloning of araA Gene Encoding L-Arabinose Isomerase from Marine Geobacillus stearothermophilus Isolated from Tanjung Api, Poso, Indonesia
title_full Cloning of araA Gene Encoding L-Arabinose Isomerase from Marine Geobacillus stearothermophilus Isolated from Tanjung Api, Poso, Indonesia
title_fullStr Cloning of araA Gene Encoding L-Arabinose Isomerase from Marine Geobacillus stearothermophilus Isolated from Tanjung Api, Poso, Indonesia
title_full_unstemmed Cloning of araA Gene Encoding L-Arabinose Isomerase from Marine Geobacillus stearothermophilus Isolated from Tanjung Api, Poso, Indonesia
title_sort cloning of araa gene encoding l-arabinose isomerase from marine geobacillus stearothermophilus isolated from tanjung api, poso, indonesia
publisher Bogor Agricultural University
series Hayati Journal of Biosciences
issn 1978-3019
2086-4094
publishDate 2010-06-01
description L-arabinose isomerase is an enzyme converting D-galactose to D-tagatose. D-tagatose is a potential sweetener-sucrose substitute which has low calorie. This research was to clone and sequence araA gene from marine bacterial strain Geobacillus stearothermophilus isolated from Tanjung Api Poso Indonesia. The amplified araA gene consisted of 1494 bp nucleotides encoding 497 amino acids. DNA alignment analysis showed that the gene had high homology with that of G. stearothermophilus T6. The enzyme had optimum activity at high temperature and alkalin condition.
topic cloning
araA gene
marine bacterium
Geobacillus stearothermophilus
url http://journal.ipb.ac.id/index.php/hayati/article/view/1348/445
work_keys_str_mv AT dewifitriani cloningofaraageneencodinglarabinoseisomerasefrommarinegeobacillusstearothermophilusisolatedfromtanjungapiposoindonesia
AT budisaksono cloningofaraageneencodinglarabinoseisomerasefrommarinegeobacillusstearothermophilusisolatedfromtanjungapiposoindonesia
_version_ 1716751125223309312

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