Serological diagnosis of dengue by an Elisa inhibition method (EIM)

An ELISA Inhibition Method (EIM) was proposed for the serologic diagnosis of dengue, comparing its results with the Hemagglutination Inhibition (HI) and the IgM capture-ELISA (MAC-ELISA). Advantages and disadvantages of both methods are discussed according to sensitivity, specificity, performance an...

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Main Authors: R. J. Fernandez, S. Vazquez
Format: Article
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 1990-09-01
Series:Memórias do Instituto Oswaldo Cruz.
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000300012
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spelling doaj-744db90fa92a411a88ad7775d077139f2020-11-25T00:17:50ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.0074-02761678-80601990-09-0185334735110.1590/S0074-02761990000300012Serological diagnosis of dengue by an Elisa inhibition method (EIM)R. J. FernandezS. VazquezAn ELISA Inhibition Method (EIM) was proposed for the serologic diagnosis of dengue, comparing its results with the Hemagglutination Inhibition (HI) and the IgM capture-ELISA (MAC-ELISA). Advantages and disadvantages of both methods are discussed according to sensitivity, specificity, performance and usefulness. As a conclusion we recommend the complementary inclusion of the EIM and MAC-ELISA substituting the HI for laboratories engaged in the diagnosis and surveillance of dengue.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000300012ELISA Inhibition MethodMAC-ELISAdengue
collection DOAJ
language English
format Article
sources DOAJ
author R. J. Fernandez
S. Vazquez
spellingShingle R. J. Fernandez
S. Vazquez
Serological diagnosis of dengue by an Elisa inhibition method (EIM)
Memórias do Instituto Oswaldo Cruz.
ELISA Inhibition Method
MAC-ELISA
dengue
author_facet R. J. Fernandez
S. Vazquez
author_sort R. J. Fernandez
title Serological diagnosis of dengue by an Elisa inhibition method (EIM)
title_short Serological diagnosis of dengue by an Elisa inhibition method (EIM)
title_full Serological diagnosis of dengue by an Elisa inhibition method (EIM)
title_fullStr Serological diagnosis of dengue by an Elisa inhibition method (EIM)
title_full_unstemmed Serological diagnosis of dengue by an Elisa inhibition method (EIM)
title_sort serological diagnosis of dengue by an elisa inhibition method (eim)
publisher Instituto Oswaldo Cruz, Ministério da Saúde
series Memórias do Instituto Oswaldo Cruz.
issn 0074-0276
1678-8060
publishDate 1990-09-01
description An ELISA Inhibition Method (EIM) was proposed for the serologic diagnosis of dengue, comparing its results with the Hemagglutination Inhibition (HI) and the IgM capture-ELISA (MAC-ELISA). Advantages and disadvantages of both methods are discussed according to sensitivity, specificity, performance and usefulness. As a conclusion we recommend the complementary inclusion of the EIM and MAC-ELISA substituting the HI for laboratories engaged in the diagnosis and surveillance of dengue.
topic ELISA Inhibition Method
MAC-ELISA
dengue
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000300012
work_keys_str_mv AT rjfernandez serologicaldiagnosisofdenguebyanelisainhibitionmethodeim
AT svazquez serologicaldiagnosisofdenguebyanelisainhibitionmethodeim
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