Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells

Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells

<p>Abstract</p> <p>Background</p> <p>Identification of secreted proteins of low abundance is often limited by abundant and high molecular weight (MW) proteins. We have optimised a procedure to overcome this limitation.</p> <p>Results</p> <p>Low M...

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Main Authors: Stephens Andrew N, Rainczuk Adam, Meehan Katie, Paule Sarah, Nie Guiying
Format: Article
Language:English
Published: BMC 2011-09-01
Series:Proteome Science
Online Access:http://www.proteomesci.com/content/9/1/50
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spelling doaj-743d392137de4ceab38d59358eeff9372020-11-25T01:26:47ZengBMCProteome Science1477-59562011-09-01915010.1186/1477-5956-9-50Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cellsStephens Andrew NRainczuk AdamMeehan KatiePaule SarahNie Guiying<p>Abstract</p> <p>Background</p> <p>Identification of secreted proteins of low abundance is often limited by abundant and high molecular weight (MW) proteins. We have optimised a procedure to overcome this limitation.</p> <p>Results</p> <p>Low MW proteins in the conditioned media of cultured cells were first captured using dual-size exclusion/affinity hydrogel nanoparticles and their identities were then revealed by proteomics.</p> <p>Conclusions</p> <p>This technique enables the analysis of secreted proteins of cultured cells low MW and low abundance.</p> http://www.proteomesci.com/content/9/1/50
collection DOAJ
language English
format Article
sources DOAJ
author Stephens Andrew N
Rainczuk Adam
Meehan Katie
Paule Sarah
Nie Guiying
spellingShingle Stephens Andrew N
Rainczuk Adam
Meehan Katie
Paule Sarah
Nie Guiying
Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells
Proteome Science
author_facet Stephens Andrew N
Rainczuk Adam
Meehan Katie
Paule Sarah
Nie Guiying
author_sort Stephens Andrew N
title Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells
title_short Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells
title_full Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells
title_fullStr Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells
title_full_unstemmed Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells
title_sort combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells
publisher BMC
series Proteome Science
issn 1477-5956
publishDate 2011-09-01
description <p>Abstract</p> <p>Background</p> <p>Identification of secreted proteins of low abundance is often limited by abundant and high molecular weight (MW) proteins. We have optimised a procedure to overcome this limitation.</p> <p>Results</p> <p>Low MW proteins in the conditioned media of cultured cells were first captured using dual-size exclusion/affinity hydrogel nanoparticles and their identities were then revealed by proteomics.</p> <p>Conclusions</p> <p>This technique enables the analysis of secreted proteins of cultured cells low MW and low abundance.</p>
url http://www.proteomesci.com/content/9/1/50
work_keys_str_mv AT stephensandrewn combinationofhydrogelnanoparticlesandproteomicstorevealsecretedproteinsassociatedwithdecidualizationofhumanuterinestromalcells
AT rainczukadam combinationofhydrogelnanoparticlesandproteomicstorevealsecretedproteinsassociatedwithdecidualizationofhumanuterinestromalcells
AT meehankatie combinationofhydrogelnanoparticlesandproteomicstorevealsecretedproteinsassociatedwithdecidualizationofhumanuterinestromalcells
AT paulesarah combinationofhydrogelnanoparticlesandproteomicstorevealsecretedproteinsassociatedwithdecidualizationofhumanuterinestromalcells
AT nieguiying combinationofhydrogelnanoparticlesandproteomicstorevealsecretedproteinsassociatedwithdecidualizationofhumanuterinestromalcells
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