An Optimized Protein Extraction Method for Gel-Free Proteomic Analysis of <i>Opuntia Ficus-Indica</i>

An Optimized Protein Extraction Method for Gel-Free Proteomic Analysis of <i>Opuntia Ficus-Indica</i>

<i>Opuntia</i> spp. is an economically important vegetable crop with high stress-tolerance and health benefits. However, proteomic analysis of the plant has been difficult due to the composition of its succulent cladodes; the abundant polysaccharides interfere with protein extraction. To...

Full description

Bibliographic Details
Main Authors:	Akiko Hashiguchi, Hisateru Yamaguchi, Keisuke Hitachi, Kazuo Watanabe
Format:	Article
Language:	English
Published:	MDPI AG 2021-01-01
Series:	Plants
Subjects:	<i>Opuntia</i> succulent protein extraction gel-free proteomic analysis abiotic stress environmental stress
Online Access:	https://www.mdpi.com/2223-7747/10/1/115

Similar Items

Biocarbon Derived from <i>Opuntia ficus indica</i> for <i>p</i>-Nitrophenol Retention
by: Hanedi Elhleli, et al.
Published: (2020-10-01)

<i>Opuntia ficus-indica</i> as an Ingredient in New Functional Pasta: Consumer Preferences in Italy
by: Nadia Palmieri, et al.
Published: (2021-04-01)

Intake of Spineless Cladodes of <i>Opuntia ficus-indica</i> During Late Pregnancy Improves Progeny Performance in Underfed Sheep
by: Venancio Cuevas Reyes, et al.
Published: (2020-06-01)

<i>Opuntia ficus-indica</i> (L.) Mill.: A Multi-Benefit Potential to Be Exploited
by: Mafalda Alexandra Silva, et al.
Published: (2021-02-01)

Calcium Bioavailability of <i>Opuntia ficus-indica</i> Cladodes in an Ovariectomized Rat Model of Postmenopausal Bone Loss
by: Michelle Quintero-García, et al.
Published: (2020-05-01)

Monitoring of Pesticides in the Cultivation of Nopal Vegetable (<i>Opuntia ficus-indica</i> (L.)) Mill, Morelos, México
by: Irene Iliana Ramírez-Bustos, et al.
Published: (2018-11-01)

Antimicrobial and Antibiofilm Activity against <i>Staphylococcus aureus</i> of <i>Opuntia ficus-indica</i> (L.) Mill. Cladode Polyphenolic Extracts
by: Federica Blando, et al.
Published: (2019-05-01)

The Effect of Juicing Methods on the Phytochemical and Antioxidant Characteristics of the Purple Prickly Pear (<i>Opuntia ficus indica</i>)—Preliminary Findings on Juice and Pomace
by: Caroline A. Gouws, et al.
Published: (2019-04-01)

An Italian Explorative Study of Willingness to Pay for a New Functional Pasta Featuring <i>Opuntia ficus indica</i>
by: Nadia Palmieri, et al.
Published: (2021-07-01)

Nutraceuticals Obtained by SFE-CO<sub>2</sub> from Cladodes of Two <i>Opuntia ficus-indica</i> (L.) Mill Wild in Calabria
by: Domenico Iacopetta, et al.
Published: (2021-01-01)

<i>Opuntia Ficus-Indica</i> L. Miller (Palma Forrageira) as an Alternative Source of Cellulose for Production of Pharmaceutical Dosage Forms and Biomaterials: Extraction and Characterization
by: Amaro César Lima de Assis, et al.
Published: (2019-07-01)

Extraction and Physicochemical Characterization of Dried Powder Mucilage from <i>Opuntia ficus-indica</i> Cladodes and Aloe Vera Leaves: A Comparative Study
by: María Carolina Otálora, et al.
Published: (2021-05-01)

Performance, Body Water Balance, Ingestive Behavior and Blood Metabolites in Goats Fed with Cactus Pear (<i>Opuntia ficus-indica</i> <i>L</i>. Miller) Silage Subjected to An Intermittent Water Supply
by: Italo Albuquerque, et al.
Published: (2020-04-01)

Phytochemistry and biological activities of Opuntia seed oils: Opuntia dillenii (Ker Gawl.) Haw. and Opuntia ficus-indica (L.) Mill. A review
by: Bouhrim Mohamed, et al.
Published: (2021-06-01)

Restoring a Degraded Rangeland: Using Fire and Herbivory to Control Opuntia Cacti Encroachment
by: Sosa, Gabriela
Published: (2011)

Calcium Bioavailability in the Soluble and Insoluble Fibers Extracted from <i>Opuntia ficus indica</i> at Different Maturity Stages in Growing Rats
by: Monsserrat Mendoza-Ávila, et al.
Published: (2020-10-01)

Profile of Volatile Aroma-Active Compounds of Cactus Seed Oil (<i>Opuntia ficus-indica</i>) from Different Locations in Morocco and Their Fate during Seed Roasting
by: Issmail Nounah, et al.
Published: (2020-09-01)

In Silico Investigation on the Interaction of Chiral Phytochemicals from <i>Opuntia ficus-indica</i> with SARS-CoV-2 M<sup>pro</sup>
by: Caterina Vicidomini, et al.
Published: (2021-06-01)

Inhibitory and Inductive Effects of <i>Opuntia ficus indica</i> Extract and Its Flavonoid Constituents on Cytochrome P450s and UDP-Glucuronosyltransferases
by: Hyesoo Jeong, et al.
Published: (2018-10-01)

Solar air drying for innovative Opuntia ficus-indica cladode dehydration
by: Ciriminna Rosaria, et al.
Published: (2019-01-01)

The use of mucilage extracted from Opuntia ficus indica as a microencapsulating shell
by: Elhleli Hanedi, et al.
Published: (2021-01-01)

Perfil fitoquímico da palma forrageira (Opuntia ficus indica) e atividade cicatrizante in vivo
by: SANTOS, Mikaella de Moura
Published: (2015)

Thermoplastic Starch Films Added with Dry Nopal (<i>Opuntia Ficus Indica</i>) Fibers
by: Fabrizio Scognamiglio, et al.
Published: (2019-11-01)

Extraction/export of nutrients in Opuntia ficus-indica under different spacings and chemical fertilizers
by: João A. da Silva, et al.
Published: (2016-03-01)

Study of carbohydrates present in the cladodes of Opuntia ficus-indica (fodder palm), according to age and season
by: Erika Maria de Oliveira Ribeiro, et al.
Published: (2010-12-01)

Pasta Supplemented with <i>Opuntia ficus-indica</i> Extract Improves Metabolic Parameters and Reduces Atherogenic Small Dense Low-Density Lipoproteins in Patients with Risk Factors for the Metabolic Syndrome: A Four-Week Intervention Study
by: Rosaria Vincenza Giglio, et al.
Published: (2020-10-01)

Spectroscopic and Structural Analyses of <i>Opuntia Robusta</i> Mucilage and Its Potential as an Edible Coating
by: Aurea Bernardino-Nicanor, et al.
Published: (2018-12-01)

Opuntia ficus-indica L. Mill Residues—Properties and Application Possibilities in Food Supplements
by: Salvador Manzur-Valdespino, et al.
Published: (2020-05-01)

Use of Opuntia ficus-indica (L.) Mill extracts from Brazilian Caatinga as an alternative of natural moisturizer in cosmetic formulations
by: Gabriel Azevedo de Brito Damasceno, et al.

Bioaccessibility, Intestinal Permeability and Plasma Stability of Isorhamnetin Glycosides from Opuntia ficus-indica (L.)
by: Marilena Antunes-Ricardo, et al.
Published: (2017-08-01)

Effect of Opuntia ficus - indica L. Fruit on Blood Glucose Level in Streptozocin Induced Diabetic Rats
by: S Kianbakht, et al.
Published: (2008-02-01)

Effect of adding mucilage from Opuntia ficus-indica and Opuntia atropes to raw milk on mesophilic aerobic bacteria and total coliforms
by: Rosa Elena Pérez Sánchez, et al.
Published: (2016-05-01)

<i>Opuntia</i> spp. Fibre Characterisation to Obtain Sustainable Materials in the Composites Field
by: Jessica Castellano, et al.
Published: (2021-06-01)

Isolation and characterization of a reserve protein from the seeds of Opuntia ficus-indica (Cactaceae)
by: A.F. Uchoa, et al.
Published: (1998-06-01)

Nopal Cactus (Opuntia ficus-indica) as a Source of Bioactive Compounds for Nutrition, Health and Disease
by: Karym El-Mostafa, et al.
Published: (2014-09-01)

Etude physico-chimique, biochimique et stabilité d’un nouveau produit : jus de cladode du figuier de Barbarie marocain (Opuntia ficus-indica et Opuntia megacantha).
by: Boutakiout, Amale
Published: (2015)

Chitosan and Opuntia ficus-indica mucilage as the base of a polymeric edible film for the protection of tomatoes against Rhizopus stolonifer
by: Dario Rafael Olicón-Hernández, et al.
Published: (2019-12-01)

Purificação, caracterização e avaliação da atividade fúngica da Lectina de Cladônias de Opuntia fícus indica
by: Maria de Sá Santana, Giselly
Published: (2014)

ELABORAÇÃO DE PRODUTOS ALIMENTÍCIOS À BASE DE PALMA (Opuntia fícus indica) E DO SEU FRUTO
by: Leyna Bezerra de Moura, et al.
Published: (2009-11-01)

Chemical Analysis of Nutritional Content of Prickly Pads (Opuntia ficus indica) at Varied Ages in an Organic Harvest
by: Mario E. Rodríguez-García, et al.
Published: (2011-04-01)