CRISPR/Cas9 mediated T7 RNA polymerase gene knock-in in E. coli BW25113 makes T7 expression system work efficiently

CRISPR/Cas9 mediated T7 RNA polymerase gene knock-in in E. coli BW25113 makes T7 expression system work efficiently

Abstract T7 Expression System is a common method of ensuring tight control and high-level induced expression. However, this system can only work in some bacterial strains in which the T7 RNA Polymerase gene resides in the chromosome. In this study, we successfully introduced a chromosomal copy of th...

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Bibliographic Details
Main Authors: Changchuan Ye, Xi Chen, Mengjie Yang, Xiangfang Zeng, Shiyan Qiao
Format: Article
Language:English
Published: BMC 2021-08-01
Series:Journal of Biological Engineering
Subjects:
E. coli
CRISPR/cas9
T7 Expression System
Fluorescent Protein
5-Aminolevulinic Acid
promoter variants
Online Access:https://doi.org/10.1186/s13036-021-00270-9

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