E-cigarettes compromise the gut barrier and trigger inflammation

Summary: E-cigarette usage continues to rise, yet the safety of e-cigarette aerosols is questioned. Using murine models of acute and chronic e-cigarette aerosol inhalation, murine colon transcriptomics, and murine and human gut-derived organoids in co-culture models, we assessed the effects of e-cig...

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Main Authors:	Aditi Sharma, Jasper Lee, Ayden G. Fonseca, Alex Moshensky, Taha Kothari, Ibrahim M. Sayed, Stella-Rita Ibeawuchi, Rama F. Pranadinata, Jason Ear, Debashis Sahoo, Laura E. Crotty-Alexander, Pradipta Ghosh, Soumita Das
Format:	Article
Language:	English
Published:	Elsevier 2021-02-01
Series:	iScience
Subjects:	Biological Sciences Physiology Toxicology Microbiology Microbiome Transcriptomics
Online Access:	http://www.sciencedirect.com/science/article/pii/S2589004221000031

id	doaj-73eae37570974f319977da6b777facb6
record_format	Article
spelling	doaj-73eae37570974f319977da6b777facb62021-02-21T04:35:01ZengElsevieriScience2589-00422021-02-01242102035E-cigarettes compromise the gut barrier and trigger inflammationAditi Sharma0Jasper Lee1Ayden G. Fonseca2Alex Moshensky3Taha Kothari4Ibrahim M. Sayed5Stella-Rita Ibeawuchi6Rama F. Pranadinata7Jason Ear8Debashis Sahoo9Laura E. Crotty-Alexander10Pradipta Ghosh11Soumita Das12Department of Pathology, University of California, San Diego, CA 92093, USADepartment of Pathology, University of California, San Diego, CA 92093, USADepartment of Cellular and Molecular Medicine, University of California, San Diego, CA 92093, USADepartment of Medicine, University of California, San Diego, CA 92093, USADepartment of Pathology, University of California, San Diego, CA 92093, USADepartment of Pathology, University of California, San Diego, CA 92093, USADepartment of Pathology, University of California, San Diego, CA 92093, USADepartment of Cellular and Molecular Medicine, University of California, San Diego, CA 92093, USADepartment of Cellular and Molecular Medicine, University of California, San Diego, CA 92093, USA; Department of Medicine, University of California, San Diego, CA 92093, USADepartment of Pediatrics, University of California, San Diego, CA 92093, USA; Department of Computer Science and Engineering, Jacobs School of Engineering, University of California, San Diego, CA 92093, USA; Rebecca and John Moore Comprehensive Cancer Center, University of California, San Diego, CA 92093, USADepartment of Medicine, University of California, San Diego, CA 92093, USA; Veterans Affairs Medical Center, VA San Diego Healthcare System, La Jolla, San Diego, CA 92093, USA; Corresponding authorDepartment of Cellular and Molecular Medicine, University of California, San Diego, CA 92093, USA; Department of Medicine, University of California, San Diego, CA 92093, USA; Rebecca and John Moore Comprehensive Cancer Center, University of California, San Diego, CA 92093, USA; Veterans Affairs Medical Center, VA San Diego Healthcare System, La Jolla, San Diego, CA 92093, USA; Corresponding authorDepartment of Pathology, University of California, San Diego, CA 92093, USA; Rebecca and John Moore Comprehensive Cancer Center, University of California, San Diego, CA 92093, USA; Corresponding authorSummary: E-cigarette usage continues to rise, yet the safety of e-cigarette aerosols is questioned. Using murine models of acute and chronic e-cigarette aerosol inhalation, murine colon transcriptomics, and murine and human gut-derived organoids in co-culture models, we assessed the effects of e-cigarette use on the gut barrier. Histologic and transcriptome analyses revealed that chronic, but not acute, nicotine-free e-cigarette use increased inflammation and reduced expression of tight junction (TJ) markers. Exposure of murine and human enteroid-derived monolayers (EDMs) to nicotine-free e-cigarette aerosols alone or in co-culture with bacteria also causes barrier disruption, downregulation of TJ protein, and enhanced inflammation in response to infection. These data highlight the harmful effects of “non-nicotine” component of e-cigarettes on the gut barrier. Considering the importance of an intact gut barrier for host fitness and the impact of gut mucosal inflammation on a multitude of chronic diseases, these findings are broadly relevant to both medicine and public health.http://www.sciencedirect.com/science/article/pii/S2589004221000031Biological SciencesPhysiologyToxicologyMicrobiologyMicrobiomeTranscriptomics
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Aditi Sharma Jasper Lee Ayden G. Fonseca Alex Moshensky Taha Kothari Ibrahim M. Sayed Stella-Rita Ibeawuchi Rama F. Pranadinata Jason Ear Debashis Sahoo Laura E. Crotty-Alexander Pradipta Ghosh Soumita Das
spellingShingle	Aditi Sharma Jasper Lee Ayden G. Fonseca Alex Moshensky Taha Kothari Ibrahim M. Sayed Stella-Rita Ibeawuchi Rama F. Pranadinata Jason Ear Debashis Sahoo Laura E. Crotty-Alexander Pradipta Ghosh Soumita Das E-cigarettes compromise the gut barrier and trigger inflammation iScience Biological Sciences Physiology Toxicology Microbiology Microbiome Transcriptomics
author_facet	Aditi Sharma Jasper Lee Ayden G. Fonseca Alex Moshensky Taha Kothari Ibrahim M. Sayed Stella-Rita Ibeawuchi Rama F. Pranadinata Jason Ear Debashis Sahoo Laura E. Crotty-Alexander Pradipta Ghosh Soumita Das
author_sort	Aditi Sharma
title	E-cigarettes compromise the gut barrier and trigger inflammation
title_short	E-cigarettes compromise the gut barrier and trigger inflammation
title_full	E-cigarettes compromise the gut barrier and trigger inflammation
title_fullStr	E-cigarettes compromise the gut barrier and trigger inflammation
title_full_unstemmed	E-cigarettes compromise the gut barrier and trigger inflammation
title_sort	e-cigarettes compromise the gut barrier and trigger inflammation
publisher	Elsevier
series	iScience
issn	2589-0042
publishDate	2021-02-01
description	Summary: E-cigarette usage continues to rise, yet the safety of e-cigarette aerosols is questioned. Using murine models of acute and chronic e-cigarette aerosol inhalation, murine colon transcriptomics, and murine and human gut-derived organoids in co-culture models, we assessed the effects of e-cigarette use on the gut barrier. Histologic and transcriptome analyses revealed that chronic, but not acute, nicotine-free e-cigarette use increased inflammation and reduced expression of tight junction (TJ) markers. Exposure of murine and human enteroid-derived monolayers (EDMs) to nicotine-free e-cigarette aerosols alone or in co-culture with bacteria also causes barrier disruption, downregulation of TJ protein, and enhanced inflammation in response to infection. These data highlight the harmful effects of “non-nicotine” component of e-cigarettes on the gut barrier. Considering the importance of an intact gut barrier for host fitness and the impact of gut mucosal inflammation on a multitude of chronic diseases, these findings are broadly relevant to both medicine and public health.
topic	Biological Sciences Physiology Toxicology Microbiology Microbiome Transcriptomics
url	http://www.sciencedirect.com/science/article/pii/S2589004221000031
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E-cigarettes compromise the gut barrier and trigger inflammation

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