Manganese suppresses oxidative stress, inflammation and caspase-3 activation in rats exposed to chlorpyrifos

The present study investigated the individual and combined impact of organophosphorus pesticide chlorpyrifos (CPF) and manganese (Mn), a naturally occurring trace metal, on hepatorenal function in adult rats. The four experimental groups namely control, CPF alone (5 mg/kg), Mn alone (10 mg/kg) and t...

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Main Authors: Solomon E. Owumi, Uche J. Dim
Format: Article
Language:English
Published: Elsevier 2019-01-01
Series:Toxicology Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2214750018307765
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spelling doaj-73e91d612c6144e3abfadb94e253fad02020-11-25T02:54:57ZengElsevierToxicology Reports2214-75002019-01-016202209Manganese suppresses oxidative stress, inflammation and caspase-3 activation in rats exposed to chlorpyrifosSolomon E. Owumi0Uche J. Dim1Corresponding author.; Cancer Research and Molecular Biology Laboratory, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, NigeriaCancer Research and Molecular Biology Laboratory, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, NigeriaThe present study investigated the individual and combined impact of organophosphorus pesticide chlorpyrifos (CPF) and manganese (Mn), a naturally occurring trace metal, on hepatorenal function in adult rats. The four experimental groups namely control, CPF alone (5 mg/kg), Mn alone (10 mg/kg) and the co-exposure group consisted of eight rats which were orally gavage for 14 consecutive days. Following sacrifice, the biomarkers of hepatorenal damage, antioxidant enzyme activities, myeloperoxidase (MPO) activity as well as levels of nitric oxide, reactive oxygen and nitrogen (RONS) species and lipid peroxidation (LPO) were analysed spectrophotometrically. Further, the concentration of tumour necrosis factor alpha (TNF-α), interleukin-1 β (IL-1β) and caspase-3 activity were assessed using ELISA. Results showed that the CPF-induced increase in biomarkers of hepatorenal toxicity were significantly (p < 0.05) alleviated in rats co-expose to CPF and Mn. Moreover, the decrease in antioxidant status as well as the elevation in RONS and LPO were significantly assuaged in rats co-treated with CPF and Mn. In addition, CPF mediated increase in TNF-α, IL-1β and caspase-3 activity were significantly diminished in the liver and kidney of rats co-exposed to CPF and Mn. Light microscopic examination evidenced that the severity of histopathological lesions induced by CPF were alleviated in rats co-exposed to CPF and Mn. In conclusion, the results highlight that co-exposure to CPF and Mn in rats assuaged CPF-induced oxidative stress, inflammation and caspase-3 activation in the liver and kidney of the rats. Keywords: Manganese, Chlorpyrifos, Co-exposure, Oxido-inflammation, Caspase-3, Ratshttp://www.sciencedirect.com/science/article/pii/S2214750018307765
collection DOAJ
language English
format Article
sources DOAJ
author Solomon E. Owumi
Uche J. Dim
spellingShingle Solomon E. Owumi
Uche J. Dim
Manganese suppresses oxidative stress, inflammation and caspase-3 activation in rats exposed to chlorpyrifos
Toxicology Reports
author_facet Solomon E. Owumi
Uche J. Dim
author_sort Solomon E. Owumi
title Manganese suppresses oxidative stress, inflammation and caspase-3 activation in rats exposed to chlorpyrifos
title_short Manganese suppresses oxidative stress, inflammation and caspase-3 activation in rats exposed to chlorpyrifos
title_full Manganese suppresses oxidative stress, inflammation and caspase-3 activation in rats exposed to chlorpyrifos
title_fullStr Manganese suppresses oxidative stress, inflammation and caspase-3 activation in rats exposed to chlorpyrifos
title_full_unstemmed Manganese suppresses oxidative stress, inflammation and caspase-3 activation in rats exposed to chlorpyrifos
title_sort manganese suppresses oxidative stress, inflammation and caspase-3 activation in rats exposed to chlorpyrifos
publisher Elsevier
series Toxicology Reports
issn 2214-7500
publishDate 2019-01-01
description The present study investigated the individual and combined impact of organophosphorus pesticide chlorpyrifos (CPF) and manganese (Mn), a naturally occurring trace metal, on hepatorenal function in adult rats. The four experimental groups namely control, CPF alone (5 mg/kg), Mn alone (10 mg/kg) and the co-exposure group consisted of eight rats which were orally gavage for 14 consecutive days. Following sacrifice, the biomarkers of hepatorenal damage, antioxidant enzyme activities, myeloperoxidase (MPO) activity as well as levels of nitric oxide, reactive oxygen and nitrogen (RONS) species and lipid peroxidation (LPO) were analysed spectrophotometrically. Further, the concentration of tumour necrosis factor alpha (TNF-α), interleukin-1 β (IL-1β) and caspase-3 activity were assessed using ELISA. Results showed that the CPF-induced increase in biomarkers of hepatorenal toxicity were significantly (p < 0.05) alleviated in rats co-expose to CPF and Mn. Moreover, the decrease in antioxidant status as well as the elevation in RONS and LPO were significantly assuaged in rats co-treated with CPF and Mn. In addition, CPF mediated increase in TNF-α, IL-1β and caspase-3 activity were significantly diminished in the liver and kidney of rats co-exposed to CPF and Mn. Light microscopic examination evidenced that the severity of histopathological lesions induced by CPF were alleviated in rats co-exposed to CPF and Mn. In conclusion, the results highlight that co-exposure to CPF and Mn in rats assuaged CPF-induced oxidative stress, inflammation and caspase-3 activation in the liver and kidney of the rats. Keywords: Manganese, Chlorpyrifos, Co-exposure, Oxido-inflammation, Caspase-3, Rats
url http://www.sciencedirect.com/science/article/pii/S2214750018307765
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