Effects of Medium Constituents on Growth and Canthinone Accumulation in Cell Suspension Cultures of Eurycoma longifolia Jack

The effect of various macronutrients, micronutrients and sucrose on growth and canthinone alkaloid production in cell suspension cultures of Pasak Bumi (Eurycoma longifolia Jack) was investigated. The optimum macronutrients and micronutrients content for the high alkaloid production of E. longifolia...

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Main Authors: LUTHFI AZIZ MAHMUD SIREGAR, CHAN LAI KENG, BOEY PENG LIM
Format: Article
Language:English
Published: Bogor Agricultural University 2009-06-01
Series:Hayati Journal of Biosciences
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S197830191630242X
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spelling doaj-73c6ad0b1be742a2bb7438423cbe73fb2020-11-24T21:49:11ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192009-06-01162697710.4308/hjb.16.2.69Effects of Medium Constituents on Growth and Canthinone Accumulation in Cell Suspension Cultures of Eurycoma longifolia JackLUTHFI AZIZ MAHMUD SIREGAR0CHAN LAI KENG1BOEY PENG LIM2Faculty of Agriculture, University of North Sumatra, Medan 20155, IndonesiaSchool of Biological Sciences, Universiti Sains Malaysia, Penang 11800, MalaysiaSchool of Chemical Sciences, Universiti Sains Malaysia, Penang 11800, MalaysiaThe effect of various macronutrients, micronutrients and sucrose on growth and canthinone alkaloid production in cell suspension cultures of Pasak Bumi (Eurycoma longifolia Jack) was investigated. The optimum macronutrients and micronutrients content for the high alkaloid production of E. longifolia Jack was different to that found in the Murashige and Skoog (MS) medium. The highest amount of alkaloids, 9-hydroxycanthin-6-one and 9-methoxycanthin-6-one, could be obtained from E. longifolia Jack cells cultured in modified MS liquid medium that containing macronutrients: 21.50 mM NH4NO3, 14.25 mM KNO3, 7.50 mM CaCl2·2H2O, 2.50 mM MgSO4·7H2O, 1.45 mM KH2PO4, while content of micronutrients was 0.233 mM FeNa-EDTA, 0.215 mM MnSO4·4H2O and without CuSO4·5H2O. Increased sucrose concentration to 4.00% (w/v) in modified MS liquid medium could increase total of two-alkaloid. The modification of macronutrients and micronutrients concentration based the optimum production of biomass was obtained MSBs medium that producing high biomass but also increasing the production of 9-hydroxycanthin-6-one. The modification of macronutrients or macronutrients and micronutrients based the optimum total of two-alkaloid was obtained MSC and MSD medium that producing low fresh weight but producing the high 9-hydroxycanthin-6-one.http://www.sciencedirect.com/science/article/pii/S197830191630242XPasak Bumi9-hydroxycanthin-6-one9-methoxycanthin-6-onemacronutrientsmicronutrientssucrose
collection DOAJ
language English
format Article
sources DOAJ
author LUTHFI AZIZ MAHMUD SIREGAR
CHAN LAI KENG
BOEY PENG LIM
spellingShingle LUTHFI AZIZ MAHMUD SIREGAR
CHAN LAI KENG
BOEY PENG LIM
Effects of Medium Constituents on Growth and Canthinone Accumulation in Cell Suspension Cultures of Eurycoma longifolia Jack
Hayati Journal of Biosciences
Pasak Bumi
9-hydroxycanthin-6-one
9-methoxycanthin-6-one
macronutrients
micronutrients
sucrose
author_facet LUTHFI AZIZ MAHMUD SIREGAR
CHAN LAI KENG
BOEY PENG LIM
author_sort LUTHFI AZIZ MAHMUD SIREGAR
title Effects of Medium Constituents on Growth and Canthinone Accumulation in Cell Suspension Cultures of Eurycoma longifolia Jack
title_short Effects of Medium Constituents on Growth and Canthinone Accumulation in Cell Suspension Cultures of Eurycoma longifolia Jack
title_full Effects of Medium Constituents on Growth and Canthinone Accumulation in Cell Suspension Cultures of Eurycoma longifolia Jack
title_fullStr Effects of Medium Constituents on Growth and Canthinone Accumulation in Cell Suspension Cultures of Eurycoma longifolia Jack
title_full_unstemmed Effects of Medium Constituents on Growth and Canthinone Accumulation in Cell Suspension Cultures of Eurycoma longifolia Jack
title_sort effects of medium constituents on growth and canthinone accumulation in cell suspension cultures of eurycoma longifolia jack
publisher Bogor Agricultural University
series Hayati Journal of Biosciences
issn 1978-3019
publishDate 2009-06-01
description The effect of various macronutrients, micronutrients and sucrose on growth and canthinone alkaloid production in cell suspension cultures of Pasak Bumi (Eurycoma longifolia Jack) was investigated. The optimum macronutrients and micronutrients content for the high alkaloid production of E. longifolia Jack was different to that found in the Murashige and Skoog (MS) medium. The highest amount of alkaloids, 9-hydroxycanthin-6-one and 9-methoxycanthin-6-one, could be obtained from E. longifolia Jack cells cultured in modified MS liquid medium that containing macronutrients: 21.50 mM NH4NO3, 14.25 mM KNO3, 7.50 mM CaCl2·2H2O, 2.50 mM MgSO4·7H2O, 1.45 mM KH2PO4, while content of micronutrients was 0.233 mM FeNa-EDTA, 0.215 mM MnSO4·4H2O and without CuSO4·5H2O. Increased sucrose concentration to 4.00% (w/v) in modified MS liquid medium could increase total of two-alkaloid. The modification of macronutrients and micronutrients concentration based the optimum production of biomass was obtained MSBs medium that producing high biomass but also increasing the production of 9-hydroxycanthin-6-one. The modification of macronutrients or macronutrients and micronutrients based the optimum total of two-alkaloid was obtained MSC and MSD medium that producing low fresh weight but producing the high 9-hydroxycanthin-6-one.
topic Pasak Bumi
9-hydroxycanthin-6-one
9-methoxycanthin-6-one
macronutrients
micronutrients
sucrose
url http://www.sciencedirect.com/science/article/pii/S197830191630242X
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