Effects of Medium Constituents on Growth and Canthinone Accumulation in Cell Suspension Cultures of Eurycoma longifolia Jack
The effect of various macronutrients, micronutrients and sucrose on growth and canthinone alkaloid production in cell suspension cultures of Pasak Bumi (Eurycoma longifolia Jack) was investigated. The optimum macronutrients and micronutrients content for the high alkaloid production of E. longifolia...
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Bogor Agricultural University
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doaj-73c6ad0b1be742a2bb7438423cbe73fb2020-11-24T21:49:11ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192009-06-01162697710.4308/hjb.16.2.69Effects of Medium Constituents on Growth and Canthinone Accumulation in Cell Suspension Cultures of Eurycoma longifolia JackLUTHFI AZIZ MAHMUD SIREGAR0CHAN LAI KENG1BOEY PENG LIM2Faculty of Agriculture, University of North Sumatra, Medan 20155, IndonesiaSchool of Biological Sciences, Universiti Sains Malaysia, Penang 11800, MalaysiaSchool of Chemical Sciences, Universiti Sains Malaysia, Penang 11800, MalaysiaThe effect of various macronutrients, micronutrients and sucrose on growth and canthinone alkaloid production in cell suspension cultures of Pasak Bumi (Eurycoma longifolia Jack) was investigated. The optimum macronutrients and micronutrients content for the high alkaloid production of E. longifolia Jack was different to that found in the Murashige and Skoog (MS) medium. The highest amount of alkaloids, 9-hydroxycanthin-6-one and 9-methoxycanthin-6-one, could be obtained from E. longifolia Jack cells cultured in modified MS liquid medium that containing macronutrients: 21.50 mM NH4NO3, 14.25 mM KNO3, 7.50 mM CaCl2·2H2O, 2.50 mM MgSO4·7H2O, 1.45 mM KH2PO4, while content of micronutrients was 0.233 mM FeNa-EDTA, 0.215 mM MnSO4·4H2O and without CuSO4·5H2O. Increased sucrose concentration to 4.00% (w/v) in modified MS liquid medium could increase total of two-alkaloid. The modification of macronutrients and micronutrients concentration based the optimum production of biomass was obtained MSBs medium that producing high biomass but also increasing the production of 9-hydroxycanthin-6-one. The modification of macronutrients or macronutrients and micronutrients based the optimum total of two-alkaloid was obtained MSC and MSD medium that producing low fresh weight but producing the high 9-hydroxycanthin-6-one.http://www.sciencedirect.com/science/article/pii/S197830191630242XPasak Bumi9-hydroxycanthin-6-one9-methoxycanthin-6-onemacronutrientsmicronutrientssucrose |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
LUTHFI AZIZ MAHMUD SIREGAR CHAN LAI KENG BOEY PENG LIM |
spellingShingle |
LUTHFI AZIZ MAHMUD SIREGAR CHAN LAI KENG BOEY PENG LIM Effects of Medium Constituents on Growth and Canthinone Accumulation in Cell Suspension Cultures of Eurycoma longifolia Jack Hayati Journal of Biosciences Pasak Bumi 9-hydroxycanthin-6-one 9-methoxycanthin-6-one macronutrients micronutrients sucrose |
author_facet |
LUTHFI AZIZ MAHMUD SIREGAR CHAN LAI KENG BOEY PENG LIM |
author_sort |
LUTHFI AZIZ MAHMUD SIREGAR |
title |
Effects of Medium Constituents on Growth and Canthinone Accumulation in Cell Suspension Cultures of Eurycoma longifolia Jack |
title_short |
Effects of Medium Constituents on Growth and Canthinone Accumulation in Cell Suspension Cultures of Eurycoma longifolia Jack |
title_full |
Effects of Medium Constituents on Growth and Canthinone Accumulation in Cell Suspension Cultures of Eurycoma longifolia Jack |
title_fullStr |
Effects of Medium Constituents on Growth and Canthinone Accumulation in Cell Suspension Cultures of Eurycoma longifolia Jack |
title_full_unstemmed |
Effects of Medium Constituents on Growth and Canthinone Accumulation in Cell Suspension Cultures of Eurycoma longifolia Jack |
title_sort |
effects of medium constituents on growth and canthinone accumulation in cell suspension cultures of eurycoma longifolia jack |
publisher |
Bogor Agricultural University |
series |
Hayati Journal of Biosciences |
issn |
1978-3019 |
publishDate |
2009-06-01 |
description |
The effect of various macronutrients, micronutrients and sucrose on growth and canthinone alkaloid production in cell suspension cultures of Pasak Bumi (Eurycoma longifolia Jack) was investigated. The optimum macronutrients and micronutrients content for the high alkaloid production of E. longifolia Jack was different to that found in the Murashige and Skoog (MS) medium. The highest amount of alkaloids, 9-hydroxycanthin-6-one and 9-methoxycanthin-6-one, could be obtained from E. longifolia Jack cells cultured in modified MS liquid medium that containing macronutrients: 21.50 mM NH4NO3, 14.25 mM KNO3, 7.50 mM CaCl2·2H2O, 2.50 mM MgSO4·7H2O, 1.45 mM KH2PO4, while content of micronutrients was 0.233 mM FeNa-EDTA, 0.215 mM MnSO4·4H2O and without CuSO4·5H2O. Increased sucrose concentration to 4.00% (w/v) in modified MS liquid medium could increase total of two-alkaloid. The modification of macronutrients and micronutrients concentration based the optimum production of biomass was obtained MSBs medium that producing high biomass but also increasing the production of 9-hydroxycanthin-6-one. The modification of macronutrients or macronutrients and micronutrients based the optimum total of two-alkaloid was obtained MSC and MSD medium that producing low fresh weight but producing the high 9-hydroxycanthin-6-one. |
topic |
Pasak Bumi 9-hydroxycanthin-6-one 9-methoxycanthin-6-one macronutrients micronutrients sucrose |
url |
http://www.sciencedirect.com/science/article/pii/S197830191630242X |
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