Subunit organisation of in vitro reconstituted HOPS and CORVET multisubunit membrane tethering complexes.

Subunit organisation of in vitro reconstituted HOPS and CORVET multisubunit membrane tethering complexes.

Biochemical and structural analysis of macromolecular protein assemblies remains challenging due to technical difficulties in recombinant expression, engineering and reconstitution of multisubunit complexes. Here we use a recently developed cell-free protein expression system based on the protozoan...

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Bibliographic Details
Main Authors: Zhong Guo, Wayne Johnston, Oleksiy Kovtun, Sergey Mureev, Cornelia Bröcker, Christian Ungermann, Kirill Alexandrov
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3846719?pdf=render

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